Literature DB >> 1415267

A new source of polymorphic DNA markers for sperm typing: analysis of microsatellite repeats in single cells.

R Hubert1, J L Weber, K Schmitt, L Zhang, N Arnheim.   

Abstract

We show that dinucleotide and tetranucleotide repeat polymorphisms can be analyzed in single cells without using radioactivity or denaturing gels. This provides a rich new source of DNA polymorphisms for genetic mapping by sperm typing. The recombination fraction between two CA repeat polymorphisms was determined after whole genome amplification of single sperm, followed by typing of two different aliquots, one aliquot for each polymorphic locus. Single-cell analysis of microsatellites may also be valuable both for preimplantation genetic disease diagnosis based on single-blastomere or polar-body analysis and for the typing of forensic or ancient DNA samples containing very small amounts of nucleic acid.

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Year:  1992        PMID： 1415267      PMCID： PMC1682826     

Source DB:  PubMed          Journal:  Am J Hum Genet        ISSN： 0002-9297            Impact factor:   11.025


  25 in total

1.  Nonradioactive assay for new microsatellite polymorphisms at the 5' end of the dystrophin gene, and estimation of intragenic recombination.

Authors:  C Oudet; R Heilig; A Hanauer; J L Mandel
Journal:  Am J Hum Genet       Date:  1991-08       Impact factor: 11.025

2.  Diagnosis of beta-thalassaemia by DNA amplification in single blastomeres from mouse preimplantation embryos.

Authors:  C Holding; M Monk
Journal:  Lancet       Date:  1989-09-02       Impact factor: 79.321

3.  Genetic analysis of DNA from single human oocytes: a model for preimplantation diagnosis of cystic fibrosis.

Authors:  C Coutelle; C Williams; A Handyside; K Hardy; R Winston; R Williamson
Journal:  BMJ       Date:  1989-07-01

4.  Amplification and analysis of DNA sequences in single human sperm and diploid cells.

Authors:  H H Li; U B Gyllensten; X F Cui; R K Saiki; H A Erlich; N Arnheim
Journal:  Nature       Date:  1988-09-29       Impact factor: 49.962

5.  Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction.

Authors:  J L Weber; P E May
Journal:  Am J Hum Genet       Date:  1989-03       Impact factor: 11.025

6.  Rapid preimplantation detection of mutant (shiverer) and normal alleles of the mouse myelin basic protein gene allowing selective implantation and birth of live young.

Authors:  C M Gomez; A L Muggleton-Harris; D G Whittingham; L E Hood; C Readhead
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 11.205

7.  Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification.

Authors:  A H Handyside; E H Kontogianni; K Hardy; R M Winston
Journal:  Nature       Date:  1990-04-19       Impact factor: 49.962

8.  Sperm typing allows accurate measurement of the recombination fraction between D3S2 and D3S3 on the short arm of human chromosome 3.

Authors:  R Hubert; V P Stanton; H Aburatani; J Warren; H Li; D E Housman; N Arnheim
Journal:  Genomics       Date:  1992-04       Impact factor: 5.736

9.  Direct electrophoretic detection of the allelic state of single DNA molecules in human sperm by using the polymerase chain reaction.

Authors:  H Li; X Cui; N Arnheim
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 11.205

10.  Towards construction of a high resolution map of the mouse genome using PCR-analysed microsatellites.

Authors:  J M Love; A M Knight; M A McAleer; J A Todd
Journal:  Nucleic Acids Res       Date:  1990-07-25       Impact factor: 16.971
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  7 in total

1.  Analysis of four microsatellite markers on the long arm of chromosome 9 by meiotic recombination in flow-sorted single sperm.

Authors:  R A Furlong; D R Goudie; N P Carter; J E Lyall; N A Affara; M A Ferguson-Smith
Journal:  Am J Hum Genet       Date:  1993-06       Impact factor: 11.025

Review 2.  The future is now: single-cell genomics of bacteria and archaea.

Authors:  Paul C Blainey
Journal:  FEMS Microbiol Rev       Date:  2013-02-11       Impact factor: 16.408

3.  Whole genome amplification of single cells from clinical peripheral blood smears.

Authors:  C P Beltinger; F Klimek; K M Debatin
Journal:  Mol Pathol       Date:  1997-10

4.  Individual variation in recombination among human males.

Authors:  J Yu; L Lazzeroni; J Qin; M M Huang; W Navidi; H Erlich; N Arnheim
Journal:  Am J Hum Genet       Date:  1996-12       Impact factor: 11.025

5.  Deletion mapping of chromosome 8p in colorectal carcinoma and dysplasia arising in ulcerative colitis, prostatic carcinoma, and malignant fibrous histiocytomas.

Authors:  M Chang; K Tsuchiya; R H Batchelor; P S Rabinovitch; B G Kulander; R C Haggitt; G C Burmer
Journal:  Am J Pathol       Date:  1994-01       Impact factor: 4.307

Review 6.  Single Cell Isolation and Analysis.

Authors:  Ping Hu; Wenhua Zhang; Hongbo Xin; Glenn Deng
Journal:  Front Cell Dev Biol       Date:  2016-10-25

Review 7.  Tools for Genomic and Transcriptomic Analysis of Microbes at Single-Cell Level.

Authors:  Zixi Chen; Lei Chen; Weiwen Zhang
Journal:  Front Microbiol       Date:  2017-09-20       Impact factor: 5.640
  7 in total

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