Literature DB >> 14131356

USE OF CALCIUM DIPICOLINATE FOR ENUMERATION OF TOTAL VIABLE ENDOSPORE POPULATIONS WITHOUT HEAT ACTIVATION.

F F BUSTA, Z J ORDAL.   

Abstract

A method, based on germination with 50 mm CaCl(2) and 40 mm sodium dipicolinate (Na(2)DPA), was developed for the determination of total viable counts of bacterial spores requiring heat activation. Incorporation of these germinating agents into Tryptone Glucose Extract Agar permitted plate-count enumeration of essentially 100% of Bacillus subtilis strain 5230 spores without a heat treatment. Other spore suspensions were surveyed for their response to CaCl(2) and Na(2)DPA, and for the subsequent removal of the heat-activation requirement for enumeration of maximal populations.

Entities:  

Keywords:  BACILLUS; BACILLUS MEGATERIUM; BACILLUS SUBTILIS; CALCIUM; EXPERIMENTAL LAB STUDY; HEAT; PYRIDINES

Mesh：

Substances：

Year:  1964        PMID： 14131356      PMCID： PMC1058077          DOI： 10.1128/am.12.2.106-110.1964

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  7 in total

1.  Effect of heat on spores of rough and smooth variants of Bacillus stearothermophilus.

Authors:  M L FIELDS
Journal:  Appl Microbiol       Date:  1963-03

2.  Heat activation and heat-induced dormancy of Bacillus stearothermophilus spores.

Authors:  N FINLEY; M L FIELDS
Journal:  Appl Microbiol       Date:  1962-05

3.  Germination of bacterial spores by long-chain alkyl amines.

Authors:  L J RODE; J W FOSTER
Journal:  Nature       Date:  1960-12-24       Impact factor: 49.962

4.  Germination of bacterial endospores with calcium and dipicolinic acid.

Authors:  H RIEMANN; Z J ORDAL
Journal:  Science       Date:  1961-05-26       Impact factor: 47.728

5.  The stimulation of germination and respiration of Bacillus megaterium spores by mangasese, L-alanine and heat.

Authors:  H S LEVINSON; M T HYATT
Journal:  J Bacteriol       Date:  1955-10       Impact factor: 3.490

6.  The activation of thermophilic spores by furfural.

Authors:  R B MEFFERED; L L CAMPBELL
Journal:  J Bacteriol       Date:  1951-07       Impact factor: 3.490

7.  Calcium dipicolinic acid-induced germination of Bacillus cereus spores.

Authors:  A KEYNAN; H O HALVORSON
Journal:  J Bacteriol       Date:  1962-01       Impact factor: 3.490
  7 in total
  7 in total

1.  Germination-induced bioluminescence, a route to determine the inhibitory effect of a combination preservation treatment on bacterial spores.

Authors:  G Ciarciaglini; P J Hill; K Davies; P J McClure; D Kilsby; M H Brown; P J Coote
Journal:  Appl Environ Microbiol       Date:  2000-09       Impact factor: 4.792

2.  Thermal inactivation characteristics of Bacillus subtilis spores at ultrahigh temperatures.

Authors:  J L Edwards; F F Busta; M L Speck
Journal:  Appl Microbiol       Date:  1965-11

3.  Distribution and correlation of events during thermal inactivation of Bacillus megaterium spores.

Authors:  H S Levinson; M T Hyatt
Journal:  J Bacteriol       Date:  1971-10       Impact factor: 3.490

4.  Heat injury of Bacillus subtilis spores at ultrahigh temperatures.

Authors:  J L Edwards; F F Busta; M L Speck
Journal:  Appl Microbiol       Date:  1965-11

5.  Inhibition of spore outgrowth and vegetative growth of Bacillus stearothermophilus by dipicolinate.

Authors:  M L Fields; H A Frank
Journal:  J Bacteriol       Date:  1973-05       Impact factor: 3.490

6.  High-throughput, single-cell analysis of macrophage interactions with fluorescently labeled Bacillus anthracis spores.

Authors:  Bojana Stojkovic; Eric M Torres; Angela M Prouty; Hetal K Patel; Lefan Zhuang; Theresa M Koehler; Jimmy D Ballard; Steven R Blanke
Journal:  Appl Environ Microbiol       Date:  2008-06-13       Impact factor: 4.792

7.  Characterization of Clostridium difficile Spores Lacking Either SpoVAC or Dipicolinic Acid Synthetase.

Authors:  M Lauren Donnelly; Kelly A Fimlaid; Aimee Shen
Journal:  J Bacteriol       Date:  2016-05-13       Impact factor: 3.490
  7 in total

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