Literature DB >> 1406067

Improved resolution of 31P nuclear magnetic resonance spectra of phospholipids from brain.

G Capuani1, T Aureli, A Miccheli, M E Di Cocco, M T Ramacci, M Delfini.   

Abstract

A method is described wherein the resolution of 31P nuclear magnetic resonance spectra of the lipophilic fraction from a Bligh-Dyer extract of rat brain can be enhanced. The lipids are dispersed as micelles in aqueous solution with sodium deoxycholate, and spectral resolution is further optimized by adjusting the pH and by removing ions from the solution. The application of the method to the study of aging in rat brain serves as an example.

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Year:  1992        PMID： 1406067     DOI： 10.1007/bf02536154

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  13 in total

1.  A rapid method of total lipid extraction and purification.

Authors:  E G BLIGH; W J DYER
Journal:  Can J Biochem Physiol       Date:  1959-08

2.  Aging brain: effect of acetyl-L-carnitine treatment on rat brain energy and phospholipid metabolism. A study by 31P and 1H NMR spectroscopy.

Authors:  T Aureli; A Miccheli; R Ricciolini; M E Di Cocco; M T Ramacci; L Angelucci; O Ghirardi; F Conti
Journal:  Brain Res       Date:  1990-08-27       Impact factor: 3.252

3.  Quantitative analysis of phospholipids by 31P-NMR.

Authors:  N Sotirhos; B Herslöf; L Kenne
Journal:  J Lipid Res       Date:  1986-04       Impact factor: 5.922

4.  Study on influence of inactivation enzyme techniques and extraction procedures on cerebral phosphorylated metabolite levels by 31P NMR spectroscopy.

Authors:  A Miccheli; T Aureli; M Delfini; M E Di Cocco; P Viola; R Gobetto; F Conti
Journal:  Cell Mol Biol       Date:  1988       Impact factor: 1.770

5.  Phospholipases in arterial tissue. 3. Phosphatide acyl-hydrolase, lysophosphatide acyl-hydrolase and sphingomyelin choline phosphohydrolase in rat and rabbit aorta in different age groups.

Authors:  S Eisenberg; Y Stein; O Stein
Journal:  Biochim Biophys Acta       Date:  1969-04-29

6.  Phosphorus NMR analysis of phospholipids in detergents.

Authors:  E London; G W Feigenson
Journal:  J Lipid Res       Date:  1979-03       Impact factor: 5.922

7.  A microanalytical technique for the quantitative determination of twenty-four classes of brain lipids.

Authors:  M A Wells; J C Dittmer
Journal:  Biochemistry       Date:  1966-11       Impact factor: 3.162

Review 8.  An update of the enzymology and regulation of sphingomyelin metabolism.

Authors:  A H Merrill; D D Jones
Journal:  Biochim Biophys Acta       Date:  1990-05-01

9.  Phosphorus NMR analysis of human white matter in mixed non-ionic detergent micelles.

Authors:  D S Marinier; R Letoublon; J Delmau
Journal:  J Lipid Res       Date:  1988-09       Impact factor: 5.922

10.  High resolution 31P NMR of extracted phospholipids.

Authors:  P Meneses; T Glonek
Journal:  J Lipid Res       Date:  1988-05       Impact factor: 5.922
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  2 in total

1.  Effect of long-term feeding with acetyl-L-carnitine on the age-related changes in rat brain lipid composition: a study by 31P NMR spectroscopy.

Authors:  T Aureli; M E Di Cocco; G Capuani; R Ricciolini; C Manetti; A Miccheli; F Conti
Journal:  Neurochem Res       Date:  2000-03       Impact factor: 3.996

2.  Meningioma phospholipid profiles measured by 31P nuclear magnetic resonance spectroscopy.

Authors:  L Seijo; T E Merchant; L T van der Ven; B D Minsky; T Glonek
Journal:  Lipids       Date:  1994-05       Impact factor: 1.880
  2 in total

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