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Literature DB >> 1404395

Preliminary crystallographic studies of urease from jack bean and from Klebsiella aerogenes.

E Jabri¹, M H Lee, R P Hausinger, P A Karplus.

Abstract

Ureases from both jack bean (Canavalia ensiformis) seeds and Klebsiella aerogenes have been crystallized by the hanging drop method. The plant-derived urease crystals are regular octahedra analogous to those obtained by Sumner. Preliminary X-ray diffraction studies show that the crystals belong to the cubic space group F4(1)32, with a = 364 A, and appear to contain one or two subunits in the asymmetric unit. Using a synchrotron source, the crystals diffract to near 3.5 A resolution. Crystals of urease from K. aerogenes belong to the cubic space group I23 or I2(1)3, with a = 170.8 A and appear to contain a single catalytic unit per asymmetric unit. The crystals diffract to better than 2.0 A resolution and are well suited for structural analysis.

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Year: 1992 PMID： 1404395 DOI： 10.1016/0022-2836(92)90232-9

Source DB: PubMed Journal: J Mol Biol ISSN： 0022-2836 Impact factor: 5.469

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Cited

12 in total

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2. Confocal microscopy on the beamline: novel three-dimensional imaging and sample positioning.

Authors: I Khan; R Gillilan; I Kriksunov; R Williams; W R Zipfel; U Englich
Journal: J Appl Crystallogr Date: 2012-09-01 Impact factor: 3.304

3. Multiple intermediate conformations of jack bean urease at low pH: anion-induced refolding.

Authors: Reshma Bhowmick; Medicherla V Jagannadham
Journal: Protein J Date: 2006-09 Impact factor: 2.371

4. Urease activity in the crystalline state.

Authors: M B Moncrief; L G Hom; E Jabri; P A Karplus; R P Hausinger
Journal: Protein Sci Date: 1995-10 Impact factor: 6.725

5. Site-directed mutagenesis of Klebsiella aerogenes urease: identification of histidine residues that appear to function in nickel ligation, substrate binding, and catalysis.

Authors: I S Park; R P Hausinger
Journal: Protein Sci Date: 1993-06 Impact factor: 6.725

10. Synthesis and evaluation of the complex-forming ability of hydroxypyranones and hydroxypyridinones with Ni (II) as possible inhibitors for urease enzyme in Helicobacter pylori.

Authors: Abbasali Palizban; Lotfollah Saghaie
Journal: Res Pharm Sci Date: 2016-07

Preliminary crystallographic studies of urease from jack bean and from Klebsiella aerogenes.

1. Slow cooling of protein crystals.

2. Confocal microscopy on the beamline: novel three-dimensional imaging and sample positioning.

3. Multiple intermediate conformations of jack bean urease at low pH: anion-induced refolding.

4. Urease activity in the crystalline state.

5. Site-directed mutagenesis of Klebsiella aerogenes urease: identification of histidine residues that appear to function in nickel ligation, substrate binding, and catalysis.

6. Purification, crystallization and preliminary X-ray analysis of urease from pigeon pea (Cajanus cajan).

Review 7. Molecular biology of microbial ureases.

8. Purification, crystallization and preliminary X-ray analysis of urease from jack bean (Canavalia ensiformis).

9. Large scale screening of commonly used Iranian traditional medicinal plants against urease activity.

10. Synthesis and evaluation of the complex-forming ability of hydroxypyranones and hydroxypyridinones with Ni (II) as possible inhibitors for urease enzyme in Helicobacter pylori.