Literature DB >> 14032458

Antibody studies in rabbit encephalomyelitis induced by a water-soluble protein fraction of rabbit cord.

R F KIBLER, A E BARNES.   

Abstract

A water-soluble protein fraction of nervous tissue was prepared by extraction of rabbit spinal cord with sodium citrate at pH 4.3. Characterization by nitrogen content and paper electrophoresis showed it to be a mixture of basic proteins. This extract demonstrated encephalitogenic activity when injected into rabbits. The most suitable technique for the measurement of serum antibody to the rabbit cord antigen proved to be the precipitation of antigen-antibody complexes by 40 per cent saturated ammonium sulfate. Antibody could not be demonstrated by the techniques of complement fixation, quantitative precipitation, and Ouchterlony plates. The early appearance of circulating antibody occurred almost exclusively in rabbits that subsequently developed EAE. Specificity of the antibodies for nervous tissue was demonstrated by appropriate blocking and adsorption experiments.

Entities:  

Keywords:  ALLERGY; ANTIBODIES; ENCEPHALOMYELITIS; NERVE TISSUE PROTEINS

Mesh：

Substances：

Year:  1962        PMID： 14032458      PMCID： PMC2137585          DOI： 10.1084/jem.116.6.807

Source DB:  PubMed          Journal:  J Exp Med        ISSN： 0022-1007            Impact factor:   14.307


  14 in total

1.  A quantitative immunochemical measure of the primary interaction between I BSA and antibody.

Authors:  R S FARR
Journal:  J Infect Dis       Date:  1958 Nov-Dec       Impact factor: 5.226

2.  An investigation into the presence of antibodies and hypersensitivity in the encephalitis produced experimentally by the injection of homologous brain suspensions.

Authors:  K R HILL
Journal:  Bull Johns Hopkins Hosp       Date:  1949-04

3.  Antigen-antibody reactions in gels.

Authors:  O OUCHTERLONY
Journal:  Acta Pathol Microbiol Scand       Date:  1949

4.  Antigen elimination from the blood as an early manifestation of the immune response.

Authors:  D W TALMAGE; F J DIXON; S C BUKANTZ; G J DAMMIN
Journal:  J Immunol       Date:  1951-10       Impact factor: 5.422

5.  Tuberculin type sensitivity to spinal cord antigen in rabbits with isoallergic encephalomyelitis.

Authors:  B H WAKSMAN; L R MORRISON
Journal:  J Immunol       Date:  1951-04       Impact factor: 5.422

6.  Determination of small quantities of nitrogen in serological precipitates and other biological materials.

Authors:  F LANNI; M L DILLON; J W BEARD
Journal:  Proc Soc Exp Biol Med       Date:  1950-05

7.  Transfedotr of Allergic Encephalomyelitis by Lymph Node Cells in Inbred Guinea Pigs.

Authors:  S H Stone
Journal:  Science       Date:  1961-09-01       Impact factor: 47.728

8.  Transfer of allergic encephalomyelitis in rats by means of lymph node cells.

Authors:  P Y PATERSON
Journal:  J Exp Med       Date:  1960-01-01       Impact factor: 14.307

9.  Critical relationships between constituents of the antigen-adjuvant emulsion affecting experimental allergic encephalomyelitis in a completely susceptible mouse genotype.

Authors:  J M LEE; H A SCHNEIDER
Journal:  J Exp Med       Date:  1962-01-01       Impact factor: 14.307

10.  Studies on acute disseminated encephalomyelitis produced experimentally in Rhesus monkeys; complement-fixing antibodies.

Authors:  C E LUMSDEN; E A KABAT; A WOLF; A E BEZER
Journal:  J Exp Med       Date:  1950-09       Impact factor: 14.307
View more
  3 in total

1.  Experimental allergic encephalomyelitis: immunological properties of encephalitogenic polypeptides and proteins.

Authors:  G Lamoureux; P R Carnegie; T A McPherson; D Johnston
Journal:  Clin Exp Immunol       Date:  1967-09       Impact factor: 4.330

2.  Agglutinating antibrain antibodies in dogs with experimental allergic encephalomyelitis.

Authors:  M Saragea; T Negru; N Rotaru; A Vladutiu
Journal:  Experientia       Date:  1965-04-15

3.  Circulating antibody to myelin basic protein in relapsing-remitting multiple sclerosis? A comparative group and sequential study by radioimmunoassay.

Authors:  J A Biggins; A Taylor; E A Caspary
Journal:  J Neurol Neurosurg Psychiatry       Date:  1978-12       Impact factor: 10.154
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.