Literature DB >> 1398743

Measurement of lymphocyte proliferation: critical analysis of radioactive and photometric methods.

H Wemme1, S Pfeifer, R Heck, J Müller-Quernheim.   

Abstract

Different methods of lymphocyte proliferation are compared to identify a non-radioactive alternative to 3H-thymidine-test. The enzymatic assays evaluating the turnover of mitochondrial dehydrogenases (MTT-test) and lysosomal hexosaminidase (NAG-test) proved not sensitive enough to substitute for 3H-thymidine incorporation. The incorporation of the nucleotide analog 5-bromodeoxyuridine (BrdU) can be exploited using an ELISA-system (enzyme linked immunosorbent assay) employing a monoclonal anti-BrdU antibody to measure cell proliferation. An optimized test protocol of the BrdU-ELISA which fulfills the requirements for a sensitive and practicable non-radioactive alternative to 3H-thymidine-test is presented.

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Year:  1992        PMID： 1398743     DOI： 10.1016/S0171-2985(11)80319-0

Source DB:  PubMed          Journal:  Immunobiology        ISSN： 0171-2985            Impact factor:   3.144


  7 in total

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Review 2.  A critical analysis of current in vitro and in vivo angiogenesis assays.

Authors:  Carolyn A Staton; Malcolm W R Reed; Nicola J Brown
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5.  Extensive sprouting of sensory afferents and hyperalgesia induced by conditional expression of nerve growth factor in the adult spinal cord.

Authors:  M I Romero; N Rangappa; L Li; E Lightfoot; M G Garry; G M Smith
Journal:  J Neurosci       Date:  2000-06-15       Impact factor: 6.167

6.  Mitogenic component in polar lipid-enriched Anaplasma phagocytophilum membranes.

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7.  Spontaneous interleukin 2 release of bronchoalveolar lavage cells in sarcoidosis is a codeterminator of prognosis.

Authors:  J Müller-Quernheim; S Pfeifer; K Kienast; G Zissel
Journal:  Lung       Date:  1996       Impact factor: 2.584
  7 in total

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