Literature DB >> 1398032

Sequence and functional analysis of the cloned Neisseria meningitidis CMP-NeuNAc synthetase.

U Edwards1, M Frosch.   

Abstract

The CMP-N-acetylneuraminic acid (CMP-NeuNAc) synthetase gene of Neisseria meningitidis group B is located on a 2.3-kb EcoRI fragment within the cps gene cluster. Nucleotide sequence determination of the gene encoding the CMP-NeuNAc synthetase revealed a 515-bp open reading frame that can encode a 18.9-kDA protein. A computer data base scan revealed a 59.4% identity to the CMP-NeuNAc synthetase gene of E. coli K1. Enzymatic activity was confirmed in vitro and in vivo. Transformation of the CMP-NeuNAc defective E. coli K1 strain EV5 with the meningococcal CMP-NeuNAc synthetase could complement the defect in E. coli.

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Year:  1992        PMID： 1398032     DOI： 10.1016/0378-1097(92)90397-7

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  8 in total

1.  Identification of a genetic locus involved in the biosynthesis of N-acetyl-D-mannosamine, a precursor of the (alpha 2-->8)-linked polysialic acid capsule of serogroup B Neisseria meningitidis.

Authors:  J S Swartley; D S Stephens
Journal:  J Bacteriol       Date:  1994-03       Impact factor: 3.490

2.  Expression of sialic acid and polysialic acid in serogroup B Neisseria meningitidis: divergent transcription of biosynthesis and transport operons through a common promoter region.

Authors:  J S Swartley; J H Ahn; L J Liu; C M Kahler; D S Stephens
Journal:  J Bacteriol       Date:  1996-07       Impact factor: 3.490

3.  Capsule switching of Neisseria meningitidis.

Authors:  J S Swartley; A A Marfin; S Edupuganti; L J Liu; P Cieslak; B Perkins; J D Wenger; D S Stephens
Journal:  Proc Natl Acad Sci U S A       Date:  1997-01-07       Impact factor: 11.205

4.  Molecular cloning and analysis of genes for sialic acid synthesis in Neisseria meningitidis group B and purification of the meningococcal CMP-NeuNAc synthetase enzyme.

Authors:  S Ganguli; G Zapata; T Wallis; C Reid; G Boulnois; W F Vann; I S Roberts
Journal:  J Bacteriol       Date:  1994-08       Impact factor: 3.490

5.  NeuA sialic acid O-acetylesterase activity modulates O-acetylation of capsular polysaccharide in group B Streptococcus.

Authors:  Amanda L Lewis; Hongzhi Cao; Silpa K Patel; Sandra Diaz; Wesley Ryan; Aaron F Carlin; Vireak Thon; Warren G Lewis; Ajit Varki; Xi Chen; Victor Nizet
Journal:  J Biol Chem       Date:  2007-07-23       Impact factor: 5.157

Review 6.  Masquerading microbial pathogens: capsular polysaccharides mimic host-tissue molecules.

Authors:  Brady F Cress; Jacob A Englaender; Wenqin He; Dennis Kasper; Robert J Linhardt; Mattheos A G Koffas
Journal:  FEMS Microbiol Rev       Date:  2014-01-27       Impact factor: 16.408

7.  Evidence for a two-metal-ion mechanism in the cytidyltransferase KdsB, an enzyme involved in lipopolysaccharide biosynthesis.

Authors:  Helgo Schmidt; Jeroen R Mesters; Jing Wu; Ronald W Woodard; Rolf Hilgenfeld; Uwe Mamat
Journal:  PLoS One       Date:  2011-08-03       Impact factor: 3.240

8.  Genetic Diversity of O-Antigens in Hafnia alvei and the Development of a Suspension Array for Serotype Detection.

Authors:  Zhifeng Duan; Tomasz Niedziela; Czeslaw Lugowski; Boyang Cao; Tianwei Wang; Lingling Xu; Baopeng Yang; Bin Liu; Lei Wang
Journal:  PLoS One       Date:  2016-05-12       Impact factor: 3.240
  8 in total

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