Literature DB >> 13930396

Fractionation of beta-glucosidases and related extracellular enzymes from Aspergillus niger.

L H LI, K W KING.   

Abstract

Industrial concentrates from Aspergillus niger culture filtrates were fractionated by ion-exchange and adsorption chromatography. Several other types of hydrolases were completely removed. Eight partially purified components were obtained. Using specific activity as an estimate of purification, one aryl-beta-glucosidase was purified 35-fold. Another component showed 147-fold purification using a viscosimetric assay with carboxymethylcellulose as substrate. The aryl-beta-glucosidase was distinctly more thermolabile than the carboxymethylcellulase.

Entities:  

Keywords:  ASPERGILLUS; GLUCOSIDASE

Mesh：

Substances：

Year:  1963        PMID： 13930396      PMCID： PMC1057995          DOI： 10.1128/am.11.4.320-325.1963

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  8 in total

1.  Fractionation of the beta-glucosidases from Aspergillus niger.

Authors:  C R KRISHNA MURTI; B A STONE
Journal:  Biochem J       Date:  1961-04       Impact factor: 3.857

2.  The production of cellulase by fungi on mixed cellulosic substrates.

Authors:  S N BASU; S N GHOSE
Journal:  Can J Microbiol       Date:  1960-06       Impact factor: 2.419

3.  Fractionation of a crude polysaccharase system by preparative paper chromatography and paper electrophoresis.

Authors:  M J WOLF; V JURKOVICH; M M MACMASTERS
Journal:  Arch Biochem Biophys       Date:  1959-03       Impact factor: 4.013

4.  On the nature of the beta-glucosidases of Myrothecium verrucaria.

Authors:  J H HASH; K W KING
Journal:  J Biol Chem       Date:  1958-05       Impact factor: 5.157

5.  The photometric microdetermination of blood glucose with glucose oxidase.

Authors:  A SAIFER; S GERSTENFELD
Journal:  J Lab Clin Med       Date:  1958-03

6.  Improved separation of sugars on the paper partition chromatogram.

Authors:  M A Jermyn; F A Isherwood
Journal:  Biochem J       Date:  1949       Impact factor: 3.857

7.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

8.  Notes on sugar determination.

Authors:  M SMOGYI
Journal:  J Biol Chem       Date:  1952-03       Impact factor: 5.157
  8 in total
  7 in total

1.  Purification and properties of a cellulase from Aspergillus niger.

Authors:  P L Hurst; J Nielsen; P A Sullivan; M G Shepherd
Journal:  Biochem J       Date:  1977-07-01       Impact factor: 3.857

2.  Distinctive properties of beta-glucosidases and related enzymes derived from a commercial Aspergillus niger cellulase.

Authors:  K W KING; R M SMIBERT
Journal:  Appl Microbiol       Date:  1963-07

3.  Trans-beta-glucosylation by alfalfa-seed enzymes.

Authors:  D H Hutson
Journal:  Biochem J       Date:  1964-07       Impact factor: 3.857

4.  Substrate specificity and mode of action of a cellulase from Aspergillus niger.

Authors:  P L Hurst; P A Sullivan; M G Shepherd
Journal:  Biochem J       Date:  1978-02-01       Impact factor: 3.857

5.  Purification and characterization of beta-glucosidase of Alcaligenes faecalis.

Authors:  Y W Han; V R Srinivasan
Journal:  J Bacteriol       Date:  1969-12       Impact factor: 3.490

6.  Inducible system for the utilization of beta-glucosides in Escherichia coli. II. Description of mutant types and genetic analysis.

Authors:  S Schaefler; W K Maas
Journal:  J Bacteriol       Date:  1967-01       Impact factor: 3.490

7.  Aspergillus oryzae and Aspergillus niger Co-Cultivation Extract Affects In Vitro Degradation, Fermentation Characteristics, and Bacterial Composition in a Diet-Specific Manner.

Authors:  Fanlin Kong; Na Lu; Yanfang Liu; Shu Zhang; Hongqin Jiang; Haomin Wang; Wei Wang; Shengli Li
Journal:  Animals (Basel)       Date:  2021-04-26       Impact factor: 2.752
  7 in total

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