Literature DB >> 1378390

The aerolysin membrane channel is formed by heptamerization of the monomer.

H U Wilmsen1, K R Leonard, W Tichelaar, J T Buckley, F Pattus.   

Abstract

The cytolytic toxin aerolysin has been found to form heptameric oligomers by SDS-PAGE electrophoresis, STEM mass measurements of single oligomers and image analysis of two-dimensional membrane crystals. Two types of crystal, flat sheets and long regular tubes, have been obtained by reconstitution of purified protein and Escherichia coli phospholipids. A noise-filtered image of the best crystalline sheets reveals a structure with 7-fold symmetry containing a central strongly stain-excluding ring that encircles a dark stain-filled channel 17 A in diameter. The ring is surrounded by seven arms each made up of two unequal sized domains. By combining projected views and side-views, a simplified model of the aerolysin channel complex has been constructed. The relevance of this structure to the mode of action of aerolysin is discussed.

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Year:  1992        PMID： 1378390      PMCID： PMC556720          DOI： 10.1002/j.1460-2075.1992.tb05310.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  20 in total

1.  Aerolysin of Aeromonas sobria: evidence for formation of ion-permeable channels and comparison with alpha-toxin of Staphylococcus aureus.

Authors:  T Chakraborty; A Schmid; S Notermans; R Benz
Journal:  Infect Immun       Date:  1990-07       Impact factor: 3.441

2.  Aerolysin, a hemolysin from Aeromonas hydrophila, forms voltage-gated channels in planar lipid bilayers.

Authors:  H U Wilmsen; F Pattus; J T Buckley
Journal:  J Membr Biol       Date:  1990-04       Impact factor: 1.843

3.  Site-directed mutagenesis at histidines of aerolysin from Aeromonas hydrophila: a lipid planar bilayer study.

Authors:  H U Wilmsen; J T Buckley; F Pattus
Journal:  Mol Microbiol       Date:  1991-11       Impact factor: 3.501

4.  Crystallization of a proform of aerolysin, a hole-forming toxin from Aeromonas hydrophila.

Authors:  A D Tucker; M W Parker; D Tsernoglou; J T Buckley
Journal:  J Mol Biol       Date:  1990-04-20       Impact factor: 5.469

5.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

6.  Membrane glycoprotein receptor and hole-forming properties of a cytolytic protein toxin.

Authors:  S P Howard; J T Buckley
Journal:  Biochemistry       Date:  1982-03-30       Impact factor: 3.162

7.  Oligomerisation of cell-bound staphylococcal alpha-toxin in relation to membrane permeabilisation.

Authors:  M Thelestam; A Olofsson; L Blomqvist; H Hebert
Journal:  Biochim Biophys Acta       Date:  1991-02-25

8.  Site-directed mutagenesis of the hole-forming toxin aerolysin: studies on the roles of histidines in receptor binding and oligomerization of the monomer.

Authors:  M J Green; J T Buckley
Journal:  Biochemistry       Date:  1990-02-27       Impact factor: 3.162

9.  Crystalline layers and three-dimensional structure of Staphylococcus aureus alpha-toxin.

Authors:  A Olofsson; U Kavéus; I Hacksell; M Thelestam; H Hebert
Journal:  J Mol Biol       Date:  1990-07-05       Impact factor: 5.469

10.  Identification and localization of high molecular weight proteins in insect flight and leg muscle.

Authors:  A Lakey; C Ferguson; S Labeit; M Reedy; A Larkins; G Butcher; K Leonard; B Bullard
Journal:  EMBO J       Date:  1990-11       Impact factor: 11.598
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  23 in total

1.  The vacuolating toxin from Helicobacter pylori forms hexameric pores in lipid bilayers at low pH.

Authors:  D M Czajkowsky; H Iwamoto; T L Cover; Z Shao
Journal:  Proc Natl Acad Sci U S A       Date:  1999-03-02       Impact factor: 11.205

2.  Decreasing Transmembrane Segment Length Greatly Decreases Perfringolysin O Pore Size.

Authors:  Qingqing Lin; Tong Wang; Huilin Li; Erwin London
Journal:  J Membr Biol       Date:  2015-04-08       Impact factor: 1.843

3.  Molecular assembly of the aerolysin pore reveals a swirling membrane-insertion mechanism.

Authors:  Matteo T Degiacomi; Ioan Iacovache; Lucile Pernot; Mohamed Chami; Misha Kudryashev; Henning Stahlberg; F Gisou van der Goot; Matteo Dal Peraro
Journal:  Nat Chem Biol       Date:  2013-08-04       Impact factor: 15.040

4.  Structural biology: Torqueing about pores.

Authors:  James C Whisstock; Michelle A Dunstone
Journal:  Nat Chem Biol       Date:  2013-10       Impact factor: 15.040

5.  Three-dimensional structure of different functional forms of the Vibrio cholerae hemolysin oligomer: a cryo-electron microscopic study.

Authors:  Somnath Dutta; Budhaditya Mazumdar; Kalyan K Banerjee; Amar N Ghosh
Journal:  J Bacteriol       Date:  2010-01       Impact factor: 3.490

Review 6.  Symmetry, stability, and dynamics of multidomain and multicomponent protein systems.

Authors:  T L Blundell; N Srinivasan
Journal:  Proc Natl Acad Sci U S A       Date:  1996-12-10       Impact factor: 11.205

7.  Optical single-channel analysis of the aerolysin pore in erythrocyte membranes.

Authors:  M Tschödrich-Rotter; U Kubitscheck; G Ugochukwu; J T Buckley; R Peters
Journal:  Biophys J       Date:  1996-02       Impact factor: 4.033

Review 8.  Obstructing toxin pathways by targeted pore blockage.

Authors:  Ekaterina M Nestorovich; Sergey M Bezrukov
Journal:  Chem Rev       Date:  2012-10-11       Impact factor: 60.622

9.  Crystal structure of Clostridium perfringens enterotoxin displays features of beta-pore-forming toxins.

Authors:  Kengo Kitadokoro; Kousuke Nishimura; Shigeki Kamitani; Aya Fukui-Miyazaki; Hirono Toshima; Hiroyuki Abe; Yoichi Kamata; Yoshiko Sugita-Konishi; Shigeki Yamamoto; Hajime Karatani; Yasuhiko Horiguchi
Journal:  J Biol Chem       Date:  2011-04-12       Impact factor: 5.157

10.  Subunit stoichiometry of staphylococcal alpha-hemolysin in crystals and on membranes: a heptameric transmembrane pore.

Authors:  J E Gouaux; O Braha; M R Hobaugh; L Song; S Cheley; C Shustak; H Bayley
Journal:  Proc Natl Acad Sci U S A       Date:  1994-12-20       Impact factor: 11.205
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