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Literature DB >> 13768661

The direct isolation of the mitotic apparatus.

D MAZIA, J M MITCHISON, H MEDINA, P HARRIS.

Abstract

A method for isolating the mitotic apparatus from dividing sea urchin eggs without the use of ethyl alcohol or of detergents is described. In the present method, the eggs are dispersed directly in a medium containing 1 M (to 1.15 M) sucrose, 0.15 M dithiodiglycol, and 0.001 M Versene at pH 6, releasing the visibly intact mitotic apparatus. The method is designed for studies of enzyme activities, lipid components, and the variables affecting the stability of the apparatus.

Entities: Chemical Species

Keywords: CELL DIVISION

Mesh：

Substances：
Sucrose

Year: 1961 PMID： 13768661 PMCID： PMC2225108 DOI： 10.1083/jcb.10.4.467

Source DB: PubMed Journal: J Biophys Biochem Cytol ISSN： 0095-9901

11 in total

1. On the physical properties of the mitotic spindle.

Authors: S INOUE
Journal: Ann N Y Acad Sci Date: 1960-10-07 Impact factor: 5.691

2. An interference microscopical and cytochemical analysis of local mass changes in the mitotic apparatus during mitosis.

Authors: R C RUSTAD
Journal: Exp Cell Res Date: 1959-03 Impact factor: 3.905

3. Studies on sulfhydryl groups during cell division of sea urchin egg. I. Glutatione.

Authors: H SAKAI; K DAN
Journal: Exp Cell Res Date: 1959-01 Impact factor: 3.905

4. Cytochemical and quantitative study of protein-bound sulfhydryl and disulfide groups in eggs of Arbacia during the first cleavage.

Authors: N KAWAMURA
Journal: Exp Cell Res Date: 1960-06 Impact factor: 3.905

5. Cell division. II. A theoretical approach to chromosomal movements and the division of the cell.

Authors: N G ANDERSON
Journal: Q Rev Biol Date: 1956-12 Impact factor: 4.875

6. [Electron microscopic study of the ultrastructure of centrioles in vertebra].

Authors: W BERNHARD; E DE HARVEN
Journal: Z Zellforsch Mikrosk Anat Date: 1956

7. SH compounds in mitosis. II. The effect of mercaptoethanol on the structure of the mitotic apparatus in sea urchin eggs.

Authors: D MAZIA; A M ZIMMERMAN
Journal: Exp Cell Res Date: 1958-08 Impact factor: 3.905

8. The Isolation and Biochemical Characterization of the Mitotic Apparatus of Dividing Cells.

Authors: D Mazia; K Dan
Journal: Proc Natl Acad Sci U S A Date: 1952-09 Impact factor: 11.205

9. Studies on the mitotic apparatus of the sea urchin by means of antigen-antibody reactions in agar.

Authors: H A WENT
Journal: J Biophys Biochem Cytol Date: 1959-12

10. A cytochemical study of the sulfhydryl groups of sea urchin eggs during the first cleavage.

Authors: N KAWAMURA; K DAN
Journal: J Biophys Biochem Cytol Date: 1958-09-25

23 in total

Review 1. Cell division screens and dynamin.

Authors: Mary Kate Bonner; Ahna R Skop
Journal: Biochem Soc Trans Date: 2008-06 Impact factor: 5.407

2. Augmentation and dispersion of the in vivo mitotic apparatus of living marine eggs.

Authors: L I Rebhun; N Sawada
Journal: Protoplasma Date: 1969 Impact factor: 3.356

3. Occurrence and properties of a thermostable protease secreted by sea-urchin embryos.

Authors: D Barrett
Journal: Biochem J Date: 1970-03 Impact factor: 3.857

4. Electrophoretic analysis of the stored histone pool in unfertilized sea urchin eggs: quantification and identification by antibody binding.

Authors: J Salik; L Herlands; H P Hoffmann; D Poccia
Journal: J Cell Biol Date: 1981-08 Impact factor: 10.539