Literature DB >> 5459944

On the reconstitution of the crystalline components of the sea urchin fertilization membrane.

J Bryan.   

Abstract

The characteristics of the reconstitution of a crystalline component of the sea urchin fertilization membrane are presented. The reassembly of large aggregates of cylindrical or tubular components is effected by the addition of calcium or other divalent cations. The reassembly requires a slightly alkaline pH and is little affected by increasing ionic strength. Reassembly is strongly inhibited by treatment with reducing agents such as dithiothreitol. The role of this protein in the formation of the fertilization membrane and its possible relation to the calcium-insoluble proteins of the mitotic apparatus are discussed.

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Year:  1970        PMID: 5459944      PMCID: PMC2107933          DOI: 10.1083/jcb.45.3.606

Source DB:  PubMed          Journal:  J Cell Biol        ISSN: 0021-9525            Impact factor:   10.539


  15 in total

1.  The isolation and preliminary characterization of a major soluble protein of the sea urchin egg.

Authors:  R E KANE; R T HERSH
Journal:  Exp Cell Res       Date:  1959-01       Impact factor: 3.905

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Fertilization membranes structure analysis with the surface replica method.

Authors:  S Inoue; J P Hardy; G H Cousineau; A K Bal
Journal:  Exp Cell Res       Date:  1967-10       Impact factor: 3.905

Review 4.  Milk proteins.

Authors:  H A McKenzie
Journal:  Adv Protein Chem       Date:  1967

5.  An analysis of acid polysaccharides produced at fertilization of sea urchin.

Authors:  K Ishihara
Journal:  Exp Cell Res       Date:  1968 Aug-Sep       Impact factor: 3.905

6.  Studies on sulfhydryl groups during cell division of sea-urchin eggs. 8. Some properties of mitotic apparatus proteins.

Authors:  H Sakai
Journal:  Biochim Biophys Acta       Date:  1966-01-04

7.  Hydrogen ion buffers for biological research.

Authors:  N E Good; G D Winget; W Winter; T N Connolly; S Izawa; R M Singh
Journal:  Biochemistry       Date:  1966-02       Impact factor: 3.162

8.  The molecular unit of the microtubules of the mitotic apparatus.

Authors:  B Kiefer; H Sakai; A J Solari; D Mazia
Journal:  J Mol Biol       Date:  1966-09       Impact factor: 5.469

9.  The isolation of a major structural element of the sea urchin fertilization membrane.

Authors:  J Bryan
Journal:  J Cell Biol       Date:  1970-03       Impact factor: 10.539

10.  A comparative study of the isolation of the cortex and the role of the calcium-insoluble protein in several species of sea urchin egg.

Authors:  R E Kane; R E Stephens
Journal:  J Cell Biol       Date:  1969-04       Impact factor: 10.539

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  7 in total

1.  Extracellular matrix modifications at fertilization: regulation of dityrosine crosslinking by transamidation.

Authors:  Julian L Wong; Gary M Wessel
Journal:  Development       Date:  2009-04-29       Impact factor: 6.868

2.  Extracellular coats on the surface of Strongylocentrotus purpuratus eggs: stereo electron microscopy of quick-frozen and deep-etched specimens.

Authors:  D E Chandler; C J Kazilek
Journal:  Cell Tissue Res       Date:  1986       Impact factor: 5.249

3.  Demonstration of the granular layer and the fate of the hyaline layer during the development of a sea urchin (Lytechinus variegatus).

Authors:  R A Cameron; N D Holland
Journal:  Cell Tissue Res       Date:  1985       Impact factor: 5.249

4.  Isolation and characterization of sea urchin egg cortical granules.

Authors:  G S Kopf; G W Moy; V D Vacquier
Journal:  J Cell Biol       Date:  1982-12       Impact factor: 10.539

5.  Sperm binding and fertilization envelope formation in a cell surface complex isolated from sea urchin eggs.

Authors:  G L Decker; W J Lennarz
Journal:  J Cell Biol       Date:  1979-04       Impact factor: 10.539

6.  Assembly of the sea urchin fertilization membrane: isolation of proteoliaisin, a calcium-dependent ovoperoxidase binding protein.

Authors:  P J Weidman; E S Kay; B M Shapiro
Journal:  J Cell Biol       Date:  1985-03       Impact factor: 10.539

7.  The vitelline layer of the sea urchin egg and its modification during fertilization. A freeze-fracture study using quick-freezing and deep-etching.

Authors:  D E Chandler; J Heuser
Journal:  J Cell Biol       Date:  1980-03       Impact factor: 10.539

  7 in total

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