Literature DB >> 1374909

Expression cloning of a rat brain somatostatin receptor cDNA.

F W Kluxen1, C Bruns, H Lübbert.   

Abstract

We have used an expression-cloning strategy to isolate a cDNA encoding a somatostatin (somatotropin release-inhibiting factor, SRIF) receptor from rat cortex and hippocampus. A positive clone was identified by autoradiography after binding of radiolabeled SRIF to COS-1 cells previously transfected with pools of cDNA clones. The deduced amino acid sequence of the receptor displays sequence and structural homology to the family of G-protein-coupled receptors. The affinity of various SRIF analogs to the expressed receptor resembles their effects on growth hormone release from pituitary cells. In addition, the distribution of the mRNA in various tissues corresponds to that described for native SRIF receptors. Therefore, we conclude that we have isolated a rat brain SRIF receptor cDNA.

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Year:  1992        PMID： 1374909      PMCID： PMC49134          DOI： 10.1073/pnas.89.10.4618

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  48 in total

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Journal:  Proc Natl Acad Sci U S A       Date:  1991-10-15       Impact factor: 11.205

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Journal:  Science       Date:  1990-10-05       Impact factor: 47.728

8.  Characterization of pituitary membrane receptors for somatostatin in the rat.

Authors:  C B Srikant; Y C Patel
Journal:  Endocrinology       Date:  1982-06       Impact factor: 4.736

9.  The somatostatin receptor on isolated pancreatic acinar cell plasma membranes. Identification of subunit structure and direct regulation by cholecystokinin.

Authors:  C Sakamoto; I D Goldfine; J A Williams
Journal:  J Biol Chem       Date:  1984-08-10       Impact factor: 5.157

10.  High level transient expression of a chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment.

Authors:  M A Lopata; D W Cleveland; B Sollner-Webb
Journal:  Nucleic Acids Res       Date:  1984-07-25       Impact factor: 16.971
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  28 in total

1.  Distribution, targeting, and internalization of the sst4 somatostatin receptor in rat brain.

Authors:  M Schreff; S Schulz; M Händel; G Keilhoff; H Braun; G Pereira; M Klutzny; H Schmidt; G Wolf; V Höllt
Journal:  J Neurosci       Date:  2000-05-15       Impact factor: 6.167

2.  Somatostatin-induced regulation of SST(2A) receptor expression and cellsurface availability in central neurons: role of receptor internalization.

Authors:  H Boudin; P Sarret; J Mazella; A Schonbrunn; A Beaudet
Journal:  J Neurosci       Date:  2000-08-15       Impact factor: 6.167

3.  Molecular cloning and functional expression of a brain-specific somatostatin receptor.

Authors:  J F Bruno; Y Xu; J Song; M Berelowitz
Journal:  Proc Natl Acad Sci U S A       Date:  1992-12-01       Impact factor: 11.205

4.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1992-07-25       Impact factor: 16.971

5.  Immunohistochemical and cytochemical localization of the somatostatin receptor subtype sst1 in the somatostatinergic parvocellular neuronal system of the rat hypothalamus.

Authors:  L Helboe; C E Stidsen; M Moller
Journal:  J Neurosci       Date:  1998-07-01       Impact factor: 6.167

6.  Expression of the somatostatin subtype 2A receptor in the rabbit retina.

Authors:  J Johnson; H Wong; J H Walsh; N C Brecha
Journal:  J Comp Neurol       Date:  1998-03-30       Impact factor: 3.215

7.  Localization of the somatostatin receptor SST2A in rat brain using a specific anti-peptide antibody.

Authors:  P Dournaud; Y Z Gu; A Schonbrunn; J Mazella; G S Tannenbaum; A Beaudet
Journal:  J Neurosci       Date:  1996-07-15       Impact factor: 6.167

Review 8.  Somatostatin.

Authors:  T Reisine
Journal:  Cell Mol Neurobiol       Date:  1995-12       Impact factor: 5.046

Review 9.  Molecular pharmacology of somatostatin receptor subtypes.

Authors:  Y C Patel
Journal:  J Endocrinol Invest       Date:  1997-06       Impact factor: 4.256

10.  Cloning and characterization of a fourth human somatostatin receptor.

Authors:  L Rohrer; F Raulf; C Bruns; R Buettner; F Hofstaedter; R Schüle
Journal:  Proc Natl Acad Sci U S A       Date:  1993-05-01       Impact factor: 11.205
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