Effect of reserpine on the 5-hydroxytryptamine and adenosinetriphosphate of the dog intestinal mucosa.

Homogenates were prepared of the mucous membrane of the small intestine from dogs previously treated with high doses of reserpine. The granular material sedimented from these homogenates was centrifuged over a density gradient. The dense fraction usually rich in adenosinetriphosphate and 5-hydroxytryptamine contained little amine and no nucleotide; on the other hand, the less dense material, believed to consist mainly of mitochondria, contained amounts of adenosinetriphosphate similar to those found in animals not treated with reserpine.