| Literature DB >> 1370026 |
G Mignot1, T Faure, V Ganne, B Arbeille, A Pavirani, J L Romet-Lemonne.
Abstract
Recombinant Chinese hamster ovary cells producing Von Willebrand factor have been successfully grown in gelatin macroporous microcarriers (Cultispher-G). Serum-free cultures were maintained in 1, 4, and 10 liter fermentors for more than two months. Comparative studies with Cytodex-3 microcarriers have been performed in 1 liter fermentors. The lower specific Von Willebrand factor productivity of CHO cells cultivated on Cultispher-G were offset by higher cell densities (10(7) - 2 x 10(7) cells/ml). Volumetric Von Willebrand factor productivity was influenced by oxygen concentration, and remained stable during scale-up from 1 to 10 liter fermentors.Entities:
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Year: 1990 PMID： 1370026 DOI： 10.1007/bf00365097
Source DB: PubMed Journal: Cytotechnology ISSN： 0920-9069 Impact factor: 2.058