Literature DB >> 1368569

Transformation of Lactobacillus helveticus subsp. jugurti with plasmid pLHR by electroporation.

H Hashiba1, R Takiguchi, S Ishii, K Aoyama.   

Abstract

Lactobacillus helveticus subsp. jugurti was transformed with plasmid, pLHR (8.5 kilobases), by electroporation. The plasmid, pLHR, consists of a cryptic plasmid, pLJ1, from L. helveticus subsp. jugurti, the Escherichia coli vector pBR329, and the erythromycin resistance gene of pAM beta 1 from Enterococcus faecalis. Maximum transformation efficiency of 1.3 x 10(4) transformants per microgram of DNA was obtained by exposure to a pulse with an exponential decay waveform at 4 kV/cm with 25 microF capacitance. The presence of glycine in the growth medium was essential for transformation. Plasmid DNA isolated from transformants had not undergone detectable rearrangements or deletions. In addition, it was found that L. helveticus subsp. jugurti has a restriction and modification system.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 1368569

Source DB:  PubMed          Journal:  Agric Biol Chem        ISSN： 0002-1369


  6 in total

1.  Incompatibility of Lactobacillus Vectors with Replicons Derived from Small Cryptic Lactobacillus Plasmids and Segregational Instability of the Introduced Vectors.

Authors:  M Posno; R J Leer; N van Luijk; M J F van Giezen; P T H M Heuvelmans; B C Lokman; P H Pouwels
Journal:  Appl Environ Microbiol       Date:  1991-06       Impact factor: 4.792

2.  Galactose utilization in Lactobacillus helveticus: isolation and characterization of the galactokinase (galK) and galactose-1-phosphate uridyl transferase (galT) genes.

Authors:  B Mollet; N Pilloud
Journal:  J Bacteriol       Date:  1991-07       Impact factor: 3.490

3.  Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids.

Authors:  Pascale Serror; Takashi Sasaki; S Dusko Ehrlich; Emmanuelle Maguin
Journal:  Appl Environ Microbiol       Date:  2002-01       Impact factor: 4.792

4.  Shuttle plasmid vectors for Lactobacillus casei and Escherichia coli with a minus origin.

Authors:  M Shimizu-Kadota; H Shibahara-Sone; H Ishiwa
Journal:  Appl Environ Microbiol       Date:  1991-11       Impact factor: 4.792

5.  Comparative sequence analysis of plasmids from Lactobacillus delbrueckii and construction of a shuttle cloning vector.

Authors:  Ju-Hoon Lee; Jamie S Halgerson; Jeong-Hwan Kim; Daniel J O'Sullivan
Journal:  Appl Environ Microbiol       Date:  2007-05-25       Impact factor: 4.792

6.  Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats.

Authors:  Xufeng Han; Lei Wang; Wei Li; Bibo Li; Yuxin Yang; Hailong Yan; Lei Qu; Yulin Chen
Journal:  Braz J Microbiol       Date:  2015-07-01       Impact factor: 2.476
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.