| Literature DB >> 1368541 |
M T Yazdi1, A Radford, J N Keen, J R Woodward.
Abstract
In studies on cellulase production by the cell-1 mutant of Neurospora crassa, eight enzymes (three exoglucanases, four endoglucanases, and one beta-glucosidase) were identified and characterized by gel filtration, ion exchange chromatography, and chromatofocusing. After purification, each of the proteins ran as a single band in polyacrylamide gel electrophoresis, using both native and denaturing gels. The molecular weights of the proteins were found to be between 70,000 and 22,000 daltons, and all were glycosylated, with carbohydrate contents ranging between 5.6% and 36%.Entities:
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Year: 1990 PMID： 1368541 DOI： 10.1016/0141-0229(90)90084-4
Source DB: PubMed Journal: Enzyme Microb Technol ISSN： 0141-0229 Impact factor: 3.493