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Literature DB >> 1368501

The effect of Pluronic F-68 on hybridoma cells in continuous culture.

Abstract

The comparative effects of Pluronic on cell growth of hybridomas were studied. The addition of 0.05% Pluronic decreased the steady-state cell number in continuous culture by about 12% compared to a non-supplemented culture. In short-term experiments, results demonstrated a gradual decrease in cell number with increasing concentration of Pluronic. Such growth inhibition was found to be a result of lowering the rate of DNA synthesis.

Entities: Chemical

Mesh：

Substances：
Poloxalene

Year: 1992 PMID： 1368501 DOI： 10.1007/bf00174201

Source DB: PubMed Journal: Appl Microbiol Biotechnol ISSN： 0175-7598 Impact factor: 4.813

6 in total

1. On the evaluation of gas-liquid interfacial effects on hybridoma viability in bubble column bioreactors.

Authors: A Handa; A N Emery; R E Spier
Journal: Dev Biol Stand Date: 1987

2. Improvements in the growth of BHK-21 cells in submerged culture.

Authors: P J Radlett; R C Telling; C J Stone; J P Whiteside
Journal: Appl Microbiol Date: 1971-10

3. Factors affecting cell growth and monoclonal antibody production in stirred reactors.

Authors: S Reuveny; D Velez; J D Macmillan; L Miller
Journal: J Immunol Methods Date: 1986-01-22 Impact factor: 2.303

4. The cultivation of animal cells at controlled dissolved oxygen partial pressure. Reprinted from Biotechnology and Bioengineering Vol. X, Issue 6, Pages 801-814 (1968).

Authors: D G Kilburn; F C Webb
Journal: Biotechnol Bioeng Date: 2000-03-20 Impact factor: 4.530

5. Pluronic polyols in human lymphocyte cell line cultures.

Authors: A Mizrahi
Journal: J Clin Microbiol Date: 1975-07 Impact factor: 5.948

6. Structural features of nonionic polyglycol polymer molecules responsible for the protective effect in sparged animal cell bioreactors.

Authors: D W Murhammer; C F Goochee
Journal: Biotechnol Prog Date: 1990 Mar-Apr

6 in total

4 in total

The effect of Pluronic F-68 on hybridoma cells in continuous culture.

1. On the evaluation of gas-liquid interfacial effects on hybridoma viability in bubble column bioreactors.

2. Improvements in the growth of BHK-21 cells in submerged culture.

3. Factors affecting cell growth and monoclonal antibody production in stirred reactors.

4. The cultivation of animal cells at controlled dissolved oxygen partial pressure. Reprinted from Biotechnology and Bioengineering Vol. X, Issue 6, Pages 801-814 (1968).

5. Pluronic polyols in human lymphocyte cell line cultures.

6. Structural features of nonionic polyglycol polymer molecules responsible for the protective effect in sparged animal cell bioreactors.

1. Specific monoclonal antibody productivity and the cell cycle-comparisons of batch, continuous and perfusion cultures.

2. Pluronic enhances the robustness and reduces the cell attachment of mammalian cells.

3. Linoleic acid improves the robustness of cells in agitated cultures.

4. Poly-γ-glutamic acid enhances the quality of recombant erythropoietin produced by CHO cells.