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Literature DB >> 1063740

Pluronic polyols in human lymphocyte cell line cultures.

Abstract

Pluronic polyols markedly improved the growth of two human lymphocyte cell lines when added to the growth medium in concentrations of 0.05 to 0.1%. The results of the current studies suggest that, in addition to the protective effect of polyols against mechanical damage of mammalian cells in submerged cultures, the pluronic compounds may also, by lowering surface tension, facilitate transport of metabolites into cells and thus increase the growth rate.

Entities: Chemical Species

Mesh：

Substances：
Alcohols
Culture Media

Year: 1975 PMID： 1063740 PMCID： PMC274118 DOI： 10.1128/jcm.2.1.11-13.1975

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

9 in total

1. Growth of L cell suspensions on a Warburg apparatus.

Authors: W S RUNYAN; R P GEYER
Journal: Proc Soc Exp Biol Med Date: 1963-04

2. Effect of pluronic F68 on growth fibroblasts in suspension on rotary shaker.

Authors: H E SWIM; R F PARKER
Journal: Proc Soc Exp Biol Med Date: 1960-01

3. Improvements in the growth of BHK-21 cells in submerged culture.

Authors: P J Radlett; R C Telling; C J Stone; J P Whiteside
Journal: Appl Microbiol Date: 1971-10

4. Culture of normal human leukocytes.

Authors: G E Moore; R E Gerner; H A Franklin
Journal: JAMA Date: 1967-02-20 Impact factor: 56.272

5. Cancer immunity--hypothesis and clinical trial of lymphocytotherapy for malignant diseases.

Authors: G E Moore; R E Gerner
Journal: Ann Surg Date: 1970-10 Impact factor: 12.969

6. Production of lymphotoxin and migration inhibitory factor by established human lymphocytic cell lines.

Authors: G A Granger; G E Moore; J G White; P Matzinger; J S Sundsmo; S Shupe; W P Kolb; J Kramer; P R Glade
Journal: J Immunol Date: 1970-06 Impact factor: 5.422

7. Anti-serum to cultured human lymphoblasts: preparation, purification and immunosuppressive properties in man.

Authors: J S Najarian; R L Simmons; H Gewurz; A Moberg; F Merkel; G E Moore
Journal: Ann Surg Date: 1969-10 Impact factor: 12.969

8. Partial substitution of serum in hematopoietic cell line media by synthetic polymers.

Authors: A Mizrahi; G E Moore
Journal: Appl Microbiol Date: 1970-06

9. Role of glycols and tweens in the production of ergot alkaloids by Claviceps paspali.

Authors: A Mizrahi; G Miller
Journal: J Bacteriol Date: 1969-03 Impact factor: 3.490

9 in total

8 in total

8. Effect of pluronic block polymers and N-acetylcysteine culture media additives on growth rate and fatty acid composition of six marine microalgae species.

Authors: Justine Sauvage; Gary H Wikfors; Xiaoxu Li; Mark Gluis; Nancy Nevejan; Koen Sabbe; Alyssa Joyce
Journal: Appl Microbiol Biotechnol Date: 2021-02-12 Impact factor: 4.813

8 in total

Pluronic polyols in human lymphocyte cell line cultures.

1. Growth of L cell suspensions on a Warburg apparatus.

2. Effect of pluronic F68 on growth fibroblasts in suspension on rotary shaker.

3. Improvements in the growth of BHK-21 cells in submerged culture.

4. Culture of normal human leukocytes.

5. Cancer immunity--hypothesis and clinical trial of lymphocytotherapy for malignant diseases.

6. Production of lymphotoxin and migration inhibitory factor by established human lymphocytic cell lines.

7. Anti-serum to cultured human lymphoblasts: preparation, purification and immunosuppressive properties in man.

8. Partial substitution of serum in hematopoietic cell line media by synthetic polymers.

9. Role of glycols and tweens in the production of ergot alkaloids by Claviceps paspali.

Review 1. Strategies for optimising serum-free media.

2. The effect of Pluronic F-68 on hybridoma cells in continuous culture.

3. Agitation and aeration effects in suspension mammalian cell cultures.

4. Media for cultivation of animal cells: an overview.

5. Medium design for insect cell culture.

6. Pluronic enhances the robustness and reduces the cell attachment of mammalian cells.

7. Effects of Pluronic F-68 on callus growth and protoplast plating efficiency of Solanum dulcamara.

8. Effect of pluronic block polymers and N-acetylcysteine culture media additives on growth rate and fatty acid composition of six marine microalgae species.