Literature DB >> 1368474

Dechlorination of pentachlorophenol by membrane bound enzymes of Rhodococcus chlorophenolicus PCP-I.

J S Uotila1, M S Salkinoja-Salonen, J H Apajalahti.   

Abstract

Dechlorination (para-hydroxylation) of pentachlorophenol (PCP) and tetrachloro-para-hydroquinone (TeCH) and O-methylation of TeCH were demonstrated in cell extracts of Rhodococcus chlorophenolicus PCP-I. PCP para-hydroxylating activity was membrane bound, whereas TeCH dechlorinating enzyme was soluble. The PCP para-hydroxylating enzyme was solubilized by Triton X-100 and the requirement for both FAD and NADPH was shown. The dechlorinating activities were inducible in contrast to the constitutive TeCH O-methylating activity. The PCP para-hydroxylation was inhibited by its product TeCH, by anoxic conditions, and by different inhibitors of P450. Participation of this cytochrome in the PCP hydroxylation was confirmed by the appearance of a carbon monoxide dependent peak of absorbance at 457 nm in the membrane fraction prepared from PCP degrading cells.

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Year:  1991        PMID： 1368474     DOI： 10.1007/bf00122422

Source DB:  PubMed          Journal:  Biodegradation        ISSN： 0923-9820            Impact factor:   3.909


  14 in total

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Authors:  A H Neilson; C Lindgren; P A Hynning; M Remberger
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Review 3.  Microbial degradation of haloaromatics.

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5.  Purification of cytochrome P-450 from n-hexadecane-grown Acinetobacter calcoaceticus.

Authors:  R Müller; O Asperger; H P Kleber
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Authors:  M M Häggblom; J H Apajalahti; M S Salkinoja-Salonen
Journal:  Appl Environ Microbiol       Date:  1988-03       Impact factor: 4.792

7.  Purification and properties of a plasmid-encoded 2,4-dichlorophenol hydroxylase.

Authors:  T Liu; P J Chapman
Journal:  FEBS Lett       Date:  1984-08-06       Impact factor: 4.124

8.  Degradation and O-methylation of chlorinated phenolic compounds by Rhodococcus and Mycobacterium strains.

Authors:  M M Häggblom; L J Nohynek; M S Salkinoja-Salonen
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9.  Enzymatic dehalogenation of pentachlorophenol by extracts from Arthrobacter sp. strain ATCC 33790.

Authors:  T Schenk; R Müller; F Mörsberger; M K Otto; F Lingens
Journal:  J Bacteriol       Date:  1989-10       Impact factor: 3.490

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Authors:  S Hartmans; M J van der Werf; J A de Bont
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  17 in total

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Authors:  D L McCarthy; A A Claude; S D Copley
Journal:  Appl Environ Microbiol       Date:  1997-05       Impact factor: 4.792

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Authors:  M Wieser; B Wagner; J Eberspächer; F Lingens
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Review 6.  Bacterial dehalogenases: biochemistry, genetics, and biotechnological applications.

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8.  Characterization of aromatic dehalogenases of Mycobacterium fortuitum CG-2.

Authors:  J S Uotila; V H Kitunen; T Saastamoinen; T Coote; M M Häggblom; M S Salkinoja-Salonen
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9.  Metabolism of halohydroquinones in Rhodococcus chlorophenolicus PCP-1.

Authors:  J S Uotila; V H Kitunen; T Coote; T Saastamoinen; M Salkinoja-Salonen; J H Apajalahti
Journal:  Biodegradation       Date:  1995-06       Impact factor: 3.909

10.  Identification of the novel hcbB operon catalyzing the dechlorination of pentachlorophenol in the Gram-positive bacterium Nocardioides sp. strain PD653.

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