Literature DB >> 1368370

RAPD analysis of Campylobacter isolates: DNA fingerprinting without the need to purify DNA.

S Mazurier1, A van de Giessen, K Heuvelman, K Wernars.   

Abstract

A method was developed to obtain reproducible DNA fingerprints from Campylobacter by PCR-based amplification, without the need to isolate total DNA. Randomly amplified polymorphic DNA (RAPD) profiles were generated with three randomly designed 10-mers, using each separately as an amplification primer. A range of C. jejuni serotypes could be typed by RAPD analysis. Depending on the primer, the analysis of RAPD profiles resulted in different levels of discrimination between the strains. Clear correlations were observed between results of RAPD analysis and serotyping. Two of the primers tested generated RAPD profiles which allowed discrimination of strains within given Penner and Lior serotypes.

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Year:  1992        PMID： 1368370     DOI： 10.1111/j.1472-765x.1992.tb00700.x

Source DB:  PubMed          Journal:  Lett Appl Microbiol        ISSN： 0266-8254            Impact factor:   2.858


  22 in total

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Authors:  T M Wassenaar; D G Newell
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2.  Allelic diversity and recombination in Campylobacter jejuni.

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3.  Identification of microorganisms using random primed PCR.

Authors:  A J Mileham
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4.  Resistotyping of campylobacters: fulfilling a need.

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5.  Factors affecting reliability and reproducibility of amplification-based DNA fingerprinting of representative bacterial pathogens.

Authors:  K D Tyler; G Wang; S D Tyler; W M Johnson
Journal:  J Clin Microbiol       Date:  1997-02       Impact factor: 5.948

6.  PCR-based characterization of Yersinia enterocolitica: comparison with biotyping and serotyping.

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Journal:  Epidemiol Infect       Date:  1995-10       Impact factor: 2.451

7.  Polymerase chain reaction-based strain characterization of noncapsulate Haemophilus influenzae.

Authors:  J Z Jordens; N I Leaves; E C Anderson; M P Slack
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8.  Genetic relatedness among environmental, clinical, and diseased-eel Vibrio vulnificus isolates from different geographic regions by ribotyping and randomly amplified polymorphic DNA PCR.

Authors:  C R Arias; M J Pujalte; E Garay; R Aznar
Journal:  Appl Environ Microbiol       Date:  1998-09       Impact factor: 4.792

9.  Characterization of verocytotoxin-producing Escherichia coli O157 isolates from patients with haemolytic uraemic syndrome in Western Europe.

Authors:  A E Heuvelink; N C van de Kar; J F Meis; L A Monnens; W J Melchers
Journal:  Epidemiol Infect       Date:  1995-08       Impact factor: 2.451

10.  Molecular subtyping by genome and plasmid analysis of Campylobacter jejuni serogroups O1 and O2 (Penner) from sporadic and outbreak cases of human diarrhoea.

Authors:  A Fayos; R J Owen; J Hernandez; C Jones; A Lastovica
Journal:  Epidemiol Infect       Date:  1993-12       Impact factor: 2.451
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