Literature DB >> 1368192

High speed automated DNA sequencing in ultrathin slab gels.

A J Kostichka1, M L Marchbanks, R L Brumley, H Drossman, L M Smith.   

Abstract

We have developed a high speed instrument for automated DNA sequence analysis. The apparatus employs laser excitation and a cooled CCD detector for the parallel detection of up to 18 sets of four fluorescently labeled DNA sequencing reactions during their electrophoretic separation in ultrathin (50-100 microns) denaturing polyacrylamide gels. Four hundred and fifty bases of sequence information is obtained from 100 ng of M13 template DNA in less than one hour, corresponding to an overall instrument throughput of over 8000 bases/hr.

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Year:  1992        PMID： 1368192     DOI： 10.1038/nbt0192-78

Source DB:  PubMed          Journal:  Biotechnology (N Y)        ISSN： 0733-222X


  8 in total

1.  A software system for data analysis in automated DNA sequencing.

Authors:  M C Giddings; J Severin; M Westphall; J Wu; L M Smith
Journal:  Genome Res       Date:  1998-06       Impact factor: 9.043

2.  High-throughput genetic analysis using microfabricated 96-sample capillary array electrophoresis microplates.

Authors:  P C Simpson; D Roach; A T Woolley; T Thorsen; R Johnston; G F Sensabaugh; R A Mathies
Journal:  Proc Natl Acad Sci U S A       Date:  1998-03-03       Impact factor: 11.205

3.  Sequencing DNA using mass spectrometry for ladder detection.

Authors:  N I Taranenko; S L Allman; V V Golovlev; N V Taranenko; N R Isola; C H Chen
Journal:  Nucleic Acids Res       Date:  1998-05-15       Impact factor: 16.971

4.  Toward fully automated genotyping: genotyping microsatellite markers by deconvolution.

Authors:  M W Perlin; G Lancia; S K Ng
Journal:  Am J Hum Genet       Date:  1995-11       Impact factor: 11.025

5.  Rapid sizing of individual fluorescently stained DNA fragments by flow cytometry.

Authors:  P M Goodwin; M E Johnson; J C Martin; W P Ambrose; B L Marrone; J H Jett; R A Keller
Journal:  Nucleic Acids Res       Date:  1993-02-25       Impact factor: 16.971

6.  DNA sequencing: modular primers assembled from a library of hexamers or pentamers.

Authors:  L E Kotler; D Zevin-Sonkin; I A Sobolev; A D Beskin; L E Ulanovsky
Journal:  Proc Natl Acad Sci U S A       Date:  1993-05-01       Impact factor: 11.205

7.  An adaptive, object oriented strategy for base calling in DNA sequence analysis.

Authors:  M C Giddings; R L Brumley; M Haker; L M Smith
Journal:  Nucleic Acids Res       Date:  1993-09-25       Impact factor: 16.971

8.  How is the Human Genome Project doing, and what have we learned so far?

Authors:  M S Guyer; F S Collins
Journal:  Proc Natl Acad Sci U S A       Date:  1995-11-21       Impact factor: 11.205
  8 in total

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