Literature DB >> 13678349

A new automated method to analyze urinary 8-hydroxydeoxyguanosine by a high-performance liquid chromatography-electrochemical detector system.

Hiroshi Kasai1.   

Abstract

A new method was developed to analyze urinary 8-hydroxydeoxyguanosine (8-OH-dG) by high-performance liquid chromatography (HPLC) coupled to an electrochemical detector (ECD). This method is unique because (i) urine is first fractionated by anion exchange chromatography (polystyrene-type resin with quaternary ammonium group, sulfate form) before analysis by reverse phase chromatography; and (ii) the 8-OH-dG fraction in the first HPLC is precisely and automatically collected based on the added ribonucleoside 8-hydroxyguanosine marker peak, which elutes 4-5 min earlier. Up to 1,000 human urine samples can be continuously analyzed with high accuracy within a few months. This method will be useful for studies in radiotherapy, molecular epidemiology, risk assessment, and health promotion.

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Year:  2003        PMID： 13678349     DOI： 10.1269/jrr.44.185

Source DB:  PubMed          Journal:  J Radiat Res        ISSN： 0449-3060            Impact factor:   2.724


  17 in total

Review 1.  8-Hydroxy-2'-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures.

Authors:  A Pilger; H W Rüdiger
Journal:  Int Arch Occup Environ Health       Date:  2006-05-10       Impact factor: 3.015

2.  Negative association between serum dioxin level and oxidative DNA damage markers in municipal waste incinerator workers.

Authors:  Jin Yoshida; Shinji Kumagai; Takeo Tabuchi; Hiroshi Kosaka; Susumu Akasaka; Hiroshi Kasai; Hajime Oda
Journal:  Int Arch Occup Environ Health       Date:  2005-09-27       Impact factor: 3.015

3.  Toward consensus in the analysis of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine as a noninvasive biomarker of oxidative stress.

Authors:  Mark D Evans; Ryszard Olinski; Steffen Loft; Marcus S Cooke
Journal:  FASEB J       Date:  2009-12-04       Impact factor: 5.191

4.  Electrochemiluminescent Array to Detect Oxidative Damage in ds-DNA Using [Os(bpy)2(phen-benz-COOH)]2+/Nafion/Graphene Films.

Authors:  Itti Bist; Boya Song; Islam M Mosa; Tia E Keyes; Aaron Martin; Robert J Forster; James F Rusling
Journal:  ACS Sens       Date:  2016-01-08       Impact factor: 7.711

5.  Immunomodulatory effects of high-protein diet with resveratrol supplementation on radiation-induced acute-phase inflammation in rats.

Authors:  Kyoung-Ok Kim; HyunJin Park; Mison Chun; Hyun-Sook Kim
Journal:  J Med Food       Date:  2014-07-23       Impact factor: 2.786

6.  Voltammetric microwell array for oxidized guanosine in intact ds-DNA.

Authors:  Boya Song; Shenmin Pan; Chi Tang; Dandan Li; James F Rusling
Journal:  Anal Chem       Date:  2013-10-28       Impact factor: 6.986

7.  Microfluidic array for simultaneous detection of DNA oxidation and DNA-adduct damage.

Authors:  Boya Song; Min Shen; Di Jiang; Spundana Malla; Islam M Mosa; Dharamainder Choudhary; James F Rusling
Journal:  Analyst       Date:  2016-08-12       Impact factor: 4.616

8.  Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine.

Authors:  Lars Barregard; Peter Møller; Trine Henriksen; Vilas Mistry; Gudrun Koppen; Pavel Rossner; Radim J Sram; Allan Weimann; Henrik E Poulsen; Robert Nataf; Roberta Andreoli; Paola Manini; Tim Marczylo; Patricia Lam; Mark D Evans; Hiroshi Kasai; Kazuaki Kawai; Yun-Shan Li; Kazuo Sakai; Rajinder Singh; Friederike Teichert; Peter B Farmer; Rafal Rozalski; Daniel Gackowski; Agnieszka Siomek; Guillermo T Saez; Concha Cerda; Karin Broberg; Christian Lindh; Mohammad Bakhtiar Hossain; Siamak Haghdoost; Chiung-Wen Hu; Mu-Rong Chao; Kuen-Yuh Wu; Hilmi Orhan; Nilufer Senduran; Raymond J Smith; Regina M Santella; Yali Su; Czarina Cortez; Susan Yeh; Ryszard Olinski; Steffen Loft; Marcus S Cooke
Journal:  Antioxid Redox Signal       Date:  2013-01-31       Impact factor: 8.401

9.  Biomarkers of nucleic acid oxidation - A summary state-of-the-art.

Authors:  Mu-Rong Chao; Mark D Evans; Chiung-Wen Hu; Yunhee Ji; Peter Møller; Pavel Rossner; Marcus S Cooke
Journal:  Redox Biol       Date:  2021-01-28       Impact factor: 11.799

10.  Sources of extracellular, oxidatively-modified DNA lesions: implications for their measurement in urine.

Authors:  Marcus S Cooke; Paul T Henderson; Mark D Evans
Journal:  J Clin Biochem Nutr       Date:  2009-10-28       Impact factor: 3.114
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