Literature DB >> 1367807

High albumin production by multicellular spheroids of adult rat hepatocytes formed in the pores of polyurethane foam.

T Matsushita1, H Ijima, N Koide, K Funatsu.   

Abstract

Adult rat hepatocytes formed spherical multicellular aggregates (spheroids) when they were cultured in the pores of polyurethane foam (PUF). The diameter of the spheroids was within the range 100-200 microns. These spheroids partly attached and immobilized in the PUF pores for at least 2 weeks. The albumin production rate by the spheroids increased up to 17.0 micrograms/10(6) nuclei per day during the first 6 days and maintained at a high level for 2 weeks. In contrast, the albumin production rate by the monolayer markedly decreased after 3 days. The spheroid culture using PUF seems to be a convenient and simple method for maintaining some differentiated functions of hepatocytes and for making a bioreactor using the function of spheroids.

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Year:  1991        PMID： 1367807     DOI： 10.1007/bf00208150

Source DB:  PubMed          Journal:  Appl Microbiol Biotechnol        ISSN： 0175-7598            Impact factor:   4.813


  8 in total

1.  Anchorage-dependent mammalian cell culture using polyurethane foam as a new substratum for cell attachment.

Authors:  T Matsushita; M Ketayama; K Kamihata; K Funatsu
Journal:  Appl Microbiol Biotechnol       Date:  1990-06       Impact factor: 4.813

2.  Production of plasminogen activator by high density culture of mammalian kidney cells.

Authors:  T Matsushita; T Ishii; Y Kawakubo; K Funatsu
Journal:  Ann N Y Acad Sci       Date:  1990       Impact factor: 5.691

Review 3.  Preparation of isolated rat liver cells.

Authors:  P O Seglen
Journal:  Methods Cell Biol       Date:  1976       Impact factor: 1.441

4.  Continued high albumin production by multicellular spheroids of adult rat hepatocytes formed in the presence of liver-derived proteoglycans.

Authors:  N Koide; T Shinji; T Tanabe; K Asano; M Kawaguchi; K Sakaguchi; Y Koide; M Mori; T Tsuji
Journal:  Biochem Biophys Res Commun       Date:  1989-05-30       Impact factor: 3.575

5.  Growth of cell-strains and primary cells on micro-carriers in homogeneous culture.

Authors:  A L van Wezel
Journal:  Nature       Date:  1967-10-07       Impact factor: 49.962

6.  The biosynthesis of rat serum albumin. VI. Intracellular transport of albumin and rates of albumin and liver protein synthesis in vivo under various physiological conditions.

Authors:  T Peters; J C Peters
Journal:  J Biol Chem       Date:  1972-06-25       Impact factor: 5.157

7.  Hepatocyte proliferation in vitro: its dependence on the use of serum-free hormonally defined medium and substrata of extracellular matrix.

Authors:  R Enat; D M Jefferson; N Ruiz-Opazo; Z Gatmaitan; L A Leinwand; L M Reid
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

8.  Plasma protein synthesis by isolated rat hepatocytes.

Authors:  L J Crane; D L Miller
Journal:  J Cell Biol       Date:  1977-01       Impact factor: 10.539
  8 in total
  11 in total

1.  Quantitative comparison of rat hepatocyte functions in two improved culture systems with or without rat liver epithelial cell line.

Authors:  Y Sakai; K Ichikawa; A Sakoda; M Suzuki
Journal:  Cytotechnology       Date:  1996-01       Impact factor: 2.058

2.  Formation of porcine hepatocyte spherical multicellular aggregates (spheroids) and analysis of drug metabolic functions.

Authors:  K Nakazawa; H Mizumoto; M Kaneko; H Ijima; T Gion; M Shimada; K Shirabe; K Takenaka; K Sugimachi; K Funatsu
Journal:  Cytotechnology       Date:  1999-09       Impact factor: 2.058

3.  Formation of cylindrical multicellular aggregate (cylindroid) and expression of liver specific functions of primary rat hepatocytes.

Authors:  H Mizumoto; M Hayakami; K Nakazawa; H Ijima; K Funatsu
Journal:  Cytotechnology       Date:  1999-09       Impact factor: 2.058

4.  Extended liver-specific functions of porcine hepatocyte spheroids entrapped in collagen gel.

Authors:  A Lazar; H J Mann; R P Remmel; R A Shatford; F B Cerra; W S Hu
Journal:  In Vitro Cell Dev Biol Anim       Date:  1995-05       Impact factor: 2.416

5.  Prolonged lidocaine metabolizing activity of primary hepatocytes with spheroid culture using polyurethane foam as a culture substratum.

Authors:  K Nakazawa; T Matsushita; K Funatsu
Journal:  Cytotechnology       Date:  1997-09       Impact factor: 2.058

6.  Stimuli-responsive microwells for formation and retrieval of cell aggregates.

Authors:  Halil Tekin; Michael Anaya; Mark D Brigham; Claire Nauman; Robert Langer; Ali Khademhosseini
Journal:  Lab Chip       Date:  2010-07-27       Impact factor: 6.799

7.  Stimulative effect of non-parenchymal liver cells on ability of tyrosine aminotransferase induction in hepatocytes.

Authors:  K Yagi; N Suenobu; M Serada; K Tsuda; A Kondoh; Y Miura
Journal:  Cytotechnology       Date:  1992       Impact factor: 2.058

8.  Intermittent addition of HGF and TGF-beta1 in rat primary hepatocyte culture.

Authors:  R Hamamoto; M Kamihira; S Iijima
Journal:  Cytotechnology       Date:  1999-09       Impact factor: 2.058

9.  Spheroid formation and functional restoration of human fetal hepatocytes on poly-amino acid-coated dishes after serial proliferation.

Authors:  Taku Matsushita; Kohji Nakano; Yasufumi Nishikura; Kenta Higuchi; Akifumi Kiyota; Ryuichi Ueoka
Journal:  Cytotechnology       Date:  2003-07       Impact factor: 2.058

10.  Bioengineering the liver: scale-up and cool chain delivery of the liver cell biomass for clinical targeting in a bioartificial liver support system.

Authors:  Eloy Erro; James Bundy; Isobel Massie; Sherri-Ann Chalmers; Aude Gautier; Spyridon Gerontas; Mike Hoare; Peter Sharratt; Sarah Choudhury; Marcin Lubowiecki; Ian Llewellyn; Cécile Legallais; Barry Fuller; Humphrey Hodgson; Clare Selden
Journal:  Biores Open Access       Date:  2013-02
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