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Literature DB >> 1367729

Optimizing protein folding to the native state in bacteria.

Abstract

A correctly folded protein is usually both active and soluble. This review focuses on novel ways to improve the folding of recombinant proteins during production in bacteria and includes a few tips for refolding proteins. Major results in correlating protein primary structure with proper folding and stability, and the production of viral antigens and antibodies in bacteria are also discussed.

Mesh：

Substances：
Recombinant Proteins

Year: 1991 PMID： 1367729 DOI： 10.1016/0958-1669(91)90046-8

Source DB: PubMed Journal: Curr Opin Biotechnol ISSN： 0958-1669 Impact factor: 9.740

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Cited

12 in total

1. Isotope labeling in insect cells.

Authors: Krishna Saxena; Arpana Dutta; Judith Klein-Seetharaman; Harald Schwalbe
Journal: Methods Mol Biol Date: 2012

2. Isolation and expression in Escherichia coli of hepB and hepC, genes coding for the glycosaminoglycan-degrading enzymes heparinase II and heparinase III, respectively, from Flavobacterium heparinum.

Authors: H Su; F Blain; R A Musil; J J Zimmermann; K Gu; D C Bennett
Journal: Appl Environ Microbiol Date: 1996-08 Impact factor: 4.792

3. Fine regulation of cI857-controlled gene expression in continuous culture of recombinant Escherichia coli by temperature.

Authors: A Villaverde; A Benito; E Viaplana; R Cubarsi
Journal: Appl Environ Microbiol Date: 1993-10 Impact factor: 4.792

4. Influenza hemagglutinin stem-fragment immunogen elicits broadly neutralizing antibodies and confers heterologous protection.

Authors: Vamsee V A Mallajosyula; Michael Citron; Francesca Ferrara; Xianghan Lu; Cheryl Callahan; Gwendolyn J Heidecker; Siddhartha P Sarma; Jessica A Flynn; Nigel J Temperton; Xiaoping Liang; Raghavan Varadarajan
Journal: Proc Natl Acad Sci U S A Date: 2014-06-09 Impact factor: 11.205

10. SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm.

Authors: Julie Lobstein; Charlie A Emrich; Chris Jeans; Melinda Faulkner; Paul Riggs; Mehmet Berkmen
Journal: Microb Cell Fact Date: 2012-05-08 Impact factor: 5.328

Optimizing protein folding to the native state in bacteria.

1. Isotope labeling in insect cells.

2. Isolation and expression in Escherichia coli of hepB and hepC, genes coding for the glycosaminoglycan-degrading enzymes heparinase II and heparinase III, respectively, from Flavobacterium heparinum.

3. Fine regulation of cI857-controlled gene expression in continuous culture of recombinant Escherichia coli by temperature.

4. Influenza hemagglutinin stem-fragment immunogen elicits broadly neutralizing antibodies and confers heterologous protection.

5. ModA and ModB, two ADP-ribosyltransferases encoded by bacteriophage T4: catalytic properties and mutation analysis.

Review 6. Strategies for achieving high-level expression of genes in Escherichia coli.

7. Cloning and expression of human colon mast cell carboxypeptidase.

8. Characterization of a progesterone-binding, three-domain antibody fragment (VH/K) expressed in Escherichia coli.

9. Sequence determinants of protein aggregation: tools to increase protein solubility.

10. SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm.