Literature DB >> 1367448

Purification, characterization, and amino terminal sequence of xylose reductase from Candida shehatae.

N W Ho1, F P Lin, S Huang, P C Andrews, G T Tsao.   

Abstract

D-Xylose is a major component of the carbohydrates derived from agricultural residues and forest products. Among more than two hundred known xylose-utilizing yeasts, only a few species are known to be able to ferment xylose anaerobically. Candida shehatae is one of such xylose-fermenting yeasts. Xylose reductase (E.C. 1.1.1.21) is a key enzyme responsible for xylose metabolism in xylose-utilizing as well as xylose-fermenting yeasts. In this paper, we report the development of a convenient and reliable procedure for the purification of xylose reductase from C. shehatae to near homogeneity. The amino acid composition and N-terminal sequence of the enzyme have also been analyzed. C. shehatae seems to contain only a single xylose reductase, but the enzyme has a dual coenzyme specificity for both NADPH and NADH. The enzyme is remarkably stable at room temperature and 4 degrees C.

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Year:  1990        PMID： 1367448     DOI： 10.1016/0141-0229(90)90177-r

Source DB:  PubMed          Journal:  Enzyme Microb Technol        ISSN： 0141-0229            Impact factor:   3.493


  9 in total

1.  Heterologous expression, purification, and characterization of a highly active xylose reductase from Neurospora crassa.

Authors:  Ryan Woodyer; Michael Simurdiak; Wilfred A van der Donk; Huimin Zhao
Journal:  Appl Environ Microbiol       Date:  2005-03       Impact factor: 4.792

2.  NAD(P)H-dependent aldose reductase from the xylose-assimilating yeast Candida tenuis. Isolation, characterization and biochemical properties of the enzyme.

Authors:  W Neuhauser; D Haltrich; K D Kulbe; B Nidetzky
Journal:  Biochem J       Date:  1997-09-15       Impact factor: 3.857

3.  Isolation of xylose reductase gene of Pichia stipitis and its expression in Saccharomyces cerevisiae.

Authors:  S Takuma; N Nakashima; M Tantirungkij; S Kinoshita; H Okada; T Seki; T Yoshida
Journal:  Appl Biochem Biotechnol       Date:  1991       Impact factor: 2.926

4.  Optimal activity and thermostability of xylose reductase from Debaryomyces hansenii UFV-170.

Authors:  Fábio C Sampaio; Janaína T de Faria; Flávia M Lopes Passos; Attilio Converti; Luis Antônio Minin
Journal:  J Ind Microbiol Biotechnol       Date:  2008-11-27       Impact factor: 3.346

5.  Cloning and characterization of the xyl1 gene, encoding an NADH-preferring xylose reductase from Candida parapsilosis, and its functional expression in Candida tropicalis.

Authors:  Jung-Kul Lee; Bong-Seong Koo; Sang-Yong Kim
Journal:  Appl Environ Microbiol       Date:  2003-10       Impact factor: 4.792

6.  Cloning, expression, and characterization of xylose reductase with higher activity from Candida tropicalis.

Authors:  Feiwei Zhang; Dairong Qiao; Hui Xu; Chong Liao; Shilin Li; Yi Cao
Journal:  J Microbiol       Date:  2009-06-26       Impact factor: 3.422

7.  Purification and partial characterization of an aldo-keto reductase from Saccharomyces cerevisiae.

Authors:  A Kuhn; C van Zyl; A van Tonder; B A Prior
Journal:  Appl Environ Microbiol       Date:  1995-04       Impact factor: 4.792

8.  Multilocus phylogenetic study of the Scheffersomyces yeast clade and characterization of the N-terminal region of xylose reductase gene.

Authors:  Hector Urbina; Meredith Blackwell
Journal:  PLoS One       Date:  2012-06-14       Impact factor: 3.240

9.  Cellulolytic enzyme expression and simultaneous conversion of lignocellulosic sugars into ethanol and xylitol by a new Candida tropicalis strain.

Authors:  Anu Jose Mattam; Arindam Kuila; Niranjan Suralikerimath; Nettem Choudary; Peddy V C Rao; Harshad Ravindra Velankar
Journal:  Biotechnol Biofuels       Date:  2016-07-26       Impact factor: 6.040
  9 in total

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