Literature DB >> 1367211

Production of human interleukin-3 using industrial microorganisms.

R W van Leen1, J G Bakhuis, R F van Beckhoven, H Burger, L C Dorssers, R W Hommes, P J Lemson, B Noordam, N L Persoon, G Wagemaker.   

Abstract

We expressed a cDNA encoding the multicolony stimulating factor interleukin-3 in a variety of cell types, including bacteria, yeast and mammalian cells. After evaluation of the advantages and disadvantages of each potential system, we designed a production and purification scheme using Bacillus licheniformis. The purification consists of hydrophobic interaction chromatography, two steps of ion exchange chromatography and gel filtration. The purified and formulated product entered clinical trials in November 1989.

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Year:  1991        PMID： 1367211     DOI： 10.1038/nbt0191-47

Source DB:  PubMed          Journal:  Biotechnology (N Y)        ISSN： 0733-222X


  7 in total

1.  Genes involved in SkfA killing factor production protect a Bacillus subtilis lipase against proteolysis.

Authors:  Helga Westers; Peter G Braun; Lidia Westers; Haike Antelmann; Michael Hecker; Jan D H Jongbloed; Hirofumi Yoshikawa; Teruo Tanaka; Jan Maarten van Dijl; Wim J Quax
Journal:  Appl Environ Microbiol       Date:  2005-04       Impact factor: 4.792

Review 2.  Protein secretion in Bacillus species.

Authors:  M Simonen; I Palva
Journal:  Microbiol Rev       Date:  1993-03

Review 3.  Signal peptide-dependent protein transport in Bacillus subtilis: a genome-based survey of the secretome.

Authors:  H Tjalsma; A Bolhuis; J D Jongbloed; S Bron; J M van Dijl
Journal:  Microbiol Mol Biol Rev       Date:  2000-09       Impact factor: 11.056

Review 4.  Merits of secretion of heterologous proteins from industrial microorganisms.

Authors:  W J Quax
Journal:  Folia Microbiol (Praha)       Date:  1997       Impact factor: 2.629

5.  Extracellular enzyme synthesis in a sporulation-deficient strain of Bacillus licheniformis.

Authors:  A B Fleming; M Tangney; P L Jørgensen; B Diderichsen; F G Priest
Journal:  Appl Environ Microbiol       Date:  1995-11       Impact factor: 4.792

6.  Acute side effects of homologous interleukin-3 in rhesus monkeys.

Authors:  F C van Gils; A H Mulder; C van den Bos; H Burger; R W van Leen; G Wagemaker
Journal:  Am J Pathol       Date:  1993-12       Impact factor: 4.307

Review 7.  Bottleneck in secretion of α-amylase in Bacillus subtilis.

Authors:  Shaomin Yan; Guang Wu
Journal:  Microb Cell Fact       Date:  2017-07-19       Impact factor: 5.328
  7 in total

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