Literature DB >> 1361963

Identification of new world Leishmania using ribosomal gene spacer probes.

P Guevara1, G Alonso, J F da Silveira, M de Mello, J V Scorza, N Añez, J L Ramírez.   

Abstract

DNA probes from the nontranscribed ribosomal spacer (NTS), of Leishmania garnhami and Leishmania braziliensis were constructed and tested for sensitivity and specificity against different Leishmania isolates. The L. garnhami probes were species-specific under hybridization conditions of high stringency, but displayed specificity for the mexicana complex under conditions of intermediate stringency. The L. braziliensis probes showed 'complex' specificity. RFLP for the nontranscribed spacer within the braziliensis complex revealed very homogeneous patterns even for organisms currently accepted as different species. A PCR assay for the detection of Leishmania from the braziliensis complex is presented.

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Year:  1992        PMID： 1361963     DOI： 10.1016/0166-6851(92)90150-i

Source DB:  PubMed          Journal:  Mol Biochem Parasitol        ISSN： 0166-6851            Impact factor:   1.759


  10 in total

1.  Rapid identification of causative species in patients with Old World leishmaniasis.

Authors:  P Minodier; R Piarroux; F Gambarelli; C Joblet; H Dumon
Journal:  J Clin Microbiol       Date:  1997-10       Impact factor: 5.948

2.  Detection of trypanosomatid Phytomonas parasitic in plants by polymerase chain reaction amplification of small subunit ribosomal DNA.

Authors:  M M Teixeira; M Campaner; E P Camargo
Journal:  Parasitol Res       Date:  1994       Impact factor: 2.289

3.  Detection and identification of Leishmania DNA within naturally infected sand flies by seminested PCR on minicircle kinetoplastic DNA.

Authors:  A M Aransay; E Scoulica; Y Tselentis
Journal:  Appl Environ Microbiol       Date:  2000-05       Impact factor: 4.792

4.  Use of PCR-restriction fragment length polymorphism analysis to identify the main new world Leishmania species and analyze their taxonomic properties and polymorphism by application of the assay to clinical samples.

Authors:  Brice Rotureau; Christophe Ravel; Pierre Couppié; Francine Pratlong; Mathieu Nacher; Jean-Pierre Dedet; Bernard Carme
Journal:  J Clin Microbiol       Date:  2006-02       Impact factor: 5.948

5.  Genetic typing reveals monomorphism between antimony sensitive and resistant Leishmania donovani isolates from visceral leishmaniasis or post kala-azar dermal leishmaniasis cases in India.

Authors:  B V Subba Raju; Srividya Gurumurthy; Katrin Kuhls; Vasundhra Bhandari; Gabriele Schnonian; Poonam Salotra
Journal:  Parasitol Res       Date:  2012-07-01       Impact factor: 2.289

Review 6.  DNA probes and PCR for diagnosis of parasitic infections.

Authors:  J B Weiss
Journal:  Clin Microbiol Rev       Date:  1995-01       Impact factor: 26.132

7.  Identification and detection of Trypanosoma cruzi by using a DNA amplification fingerprint obtained from the ribosomal intergenic spacer.

Authors:  N González; I Galindo; P Guevara; E Novak; J V Scorza; N Añez; J F Da Silveira; J L Ramírez
Journal:  J Clin Microbiol       Date:  1994-01       Impact factor: 5.948

8.  Presence of Leishmania braziliensis in blood samples from cured patients or at different stages of immunotherapy.

Authors:  P Guevara; E Rojas; N Gonzalez; J V Scorza; N Añez; M Valera; J L Ramírez
Journal:  Clin Diagn Lab Immunol       Date:  1994-07

9.  Single-step multiplex PCR assay for characterization of New World Leishmania complexes.

Authors:  E Harris; G Kropp; A Belli; B Rodriguez; N Agabian
Journal:  J Clin Microbiol       Date:  1998-07       Impact factor: 5.948

Review 10.  Detection of animal pathogens by using the polymerase chain reaction (PCR).

Authors:  J M Rodriguez
Journal:  Vet J       Date:  1997-05       Impact factor: 2.688
  10 in total

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