Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Some technical parameters influencing the precision and accuracy of fragment size determination for RFLP's.

Literature DB >> 1354481

Some technical parameters influencing the precision and accuracy of fragment size determination for RFLP's.

Abstract

A comparison was carried out between 3 computer-assisted systems for the estimation of DNA fragment length: (1) the "Digitab" system (developed at our institute), (2) the FBI analysis system (FBI Quantico, USA) and (3) the BioImage system (Waters/Millipore, USA). Both the FBI system and the BioImage system were found to be much more precise than the manual Digitab system. In an additional experiment, a possible influence of the field strength on the "accuracy" of fragment size measurement was checked. Lowering the field strength to approx. 1V/cm led to a statistically significant increase in the measured fragment size.

Mesh：

Year: 1992 PMID： 1354481 DOI： 10.1007/bf01371234

Source DB: PubMed Journal: Int J Legal Med ISSN： 0937-9827 Impact factor: 2.686

Keyword Cloud
References

11 in total

1. Report of a European collaborative exercise comparing DNA typing results using a single locus VNTR probe.

Authors: P M Schneider; R Fimmers; S Woodroffe; D J Werrett; W Bär; B Brinkmann; B Eriksen; S Jones; A D Kloosterman; B Mevåg
Journal: Forensic Sci Int Date: 1991 Jan-Feb Impact factor: 2.395

2. A report of an international collaborative experiment to demonstrate the uniformity obtainable using DNA profiling techniques.

Authors: P Gill; S Woodroffe; W Bär; B Brinkmann; A Carracedo; B Eriksen; S Jones; A D Kloosterman; B Ludes; B Mevag
Journal: Forensic Sci Int Date: 1992-02 Impact factor: 2.395

7. Role of molecular conformation in determining the electrophoretic properties of polynucleotides in agarose-acrylamide composite gels.

Authors: M P Fisher; C W Dingman
Journal: Biochemistry Date: 1971-05-11 Impact factor: 3.162

Some technical parameters influencing the precision and accuracy of fragment size determination for RFLP's.

1. Report of a European collaborative exercise comparing DNA typing results using a single locus VNTR probe.

2. A report of an international collaborative experiment to demonstrate the uniformity obtainable using DNA profiling techniques.

3. Population and family data of RFLP's using selected single- and multi-locus systems.

4. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

5. Variable number of tandem repeat (VNTR) markers for human gene mapping.

6. Hypervariable 'minisatellite' regions in human DNA.

7. Role of molecular conformation in determining the electrophoretic properties of polynucleotides in agarose-acrylamide composite gels.

8. Improved estimation of DNA fragment lengths from Agarose gels.

9. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

10. Forensic application of DNA 'fingerprints'.