Literature DB >> 1352873

Purification of PCNA as a nucleotide excision repair protein.

A F Nichols1, A Sancar.   

Abstract

Human cell free extracts carry out nucleotide excision repair in vitro. The extract is readily separated into two fractions by chromatography on a DEAE column. Neither the low salt (0.1 M KCl) nor the high salt (0.8 M KCl) fractions are capable of repair synthesis but the combination of the two restore the repair synthesis activity. Using the repair synthesis assay we purified a protein of 37 kDa from the high salt fraction which upon addition to the low salt fraction restores repair synthesis activity. Amino acid sequence analysis, amino acid composition and immunoblotting with PCNA antibodies revealed that the 37 kDa protein is the proliferating cell nuclear antigen (PCNA) known to stimulate DNA Polymerases delta and epsilon. By using an assay which specifically measures the excision of thymine dimers we found that PCNA is not required for the actual excision reaction per se but increases the extent of excision by enabling the excision repair enzyme to turn over catalytically.

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Year:  1992        PMID： 1352873      PMCID： PMC312376          DOI： 10.1093/nar/20.10.2441

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  32 in total

1.  Sequential initiation of lagging and leading strand synthesis by two different polymerase complexes at the SV40 DNA replication origin.

Authors:  T Tsurimoto; T Melendy; B Stillman
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2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
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3.  Effect of DNA polymerase I and DNA helicase II on the turnover rate of UvrABC excision nuclease.

Authors:  I Husain; B Van Houten; D C Thomas; M Abdel-Monem; A Sancar
Journal:  Proc Natl Acad Sci U S A       Date:  1985-10       Impact factor: 11.205

4.  Synthesis of DNA by DNA polymerase epsilon in vitro.

Authors:  S H Lee; Z Q Pan; A D Kwong; P M Burgers; J Hurwitz
Journal:  J Biol Chem       Date:  1991-11-25       Impact factor: 5.157

5.  Substrate overlap and functional competition between human nucleotide excision repair and Escherichia coli photolyase and (a)BC excision nuclease.

Authors:  A Sancar
Journal:  Biochemistry       Date:  1990-06-19       Impact factor: 3.162

6.  Human nucleotide excision repair in vitro: repair of pyrimidine dimers, psoralen and cisplatin adducts by HeLa cell-free extract.

Authors:  I Husain; W Carlton; A Sancar
Journal:  Nucleic Acids Res       Date:  1989-06-26       Impact factor: 16.971

7.  Human nucleotide excision nuclease removes thymine dimers from DNA by incising the 22nd phosphodiester bond 5' and the 6th phosphodiester bond 3' to the photodimer.

Authors:  J C Huang; D L Svoboda; J T Reardon; A Sancar
Journal:  Proc Natl Acad Sci U S A       Date:  1992-04-15       Impact factor: 11.205

8.  Increased nuclear cyclin/PCNA antigen staining of non S-phase transformed human amnion cells engaged in nucleotide excision DNA repair.

Authors:  J E Celis; P Madsen
Journal:  FEBS Lett       Date:  1986-12-15       Impact factor: 4.124

9.  Requirement for the replication protein SSB in human DNA excision repair.

Authors:  D Coverley; M K Kenny; M Munn; W D Rupp; D P Lane; R D Wood
Journal:  Nature       Date:  1991-02-07       Impact factor: 49.962

10.  Complementation of DNA repair in xeroderma pigmentosum group A cell extracts by a protein with affinity for damaged DNA.

Authors:  P Robins; C J Jones; M Biggerstaff; T Lindahl; R D Wood
Journal:  EMBO J       Date:  1991-12       Impact factor: 11.598
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  52 in total

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3.  Mutations in yeast proliferating cell nuclear antigen define distinct sites for interaction with DNA polymerase delta and DNA polymerase epsilon.

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4.  Coordination of dual incision and repair synthesis in human nucleotide excision repair.

Authors:  Lidija Staresincic; Adebanke F Fagbemi; Jacqueline H Enzlin; Audrey M Gourdin; Nils Wijgers; Isabelle Dunand-Sauthier; Giuseppina Giglia-Mari; Stuart G Clarkson; Wim Vermeulen; Orlando D Schärer
Journal:  EMBO J       Date:  2009-03-12       Impact factor: 11.598

5.  DNA polymerase epsilon may be dispensable for SV40- but not cellular-DNA replication.

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6.  PIDD orchestrates translesion DNA synthesis in response to UV irradiation.

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Review 7.  Dynamic interrelationships between DNA replication, methylation, and repair.

Authors:  K D Robertson; P A Jones
Journal:  Am J Hum Genet       Date:  1997-12       Impact factor: 11.025

8.  Incubation at the nonpermissive temperature induces deficiencies in UV resistance and mutagenesis in mouse mutant cells expressing a temperature-sensitive ubiquitin-activating enzyme (E1).

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Journal:  Mol Cell Biol       Date:  1997-03       Impact factor: 4.272

9.  Properties of damage-dependent DNA incision by nucleotide excision repair in human cell-free extracts.

Authors:  P Calsou; B Salles
Journal:  Nucleic Acids Res       Date:  1994-11-25       Impact factor: 16.971

10.  PCNA binding proteins in Drosophila melanogaster : the analysis of a conserved PCNA binding domain.

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Journal:  Nucleic Acids Res       Date:  1998-09-01       Impact factor: 16.971
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