| Literature DB >> 1347503 |
P Muzzin1, J P Revelli, C M Fraser, J P Giacobino.
Abstract
Two populations of [3H]CGP 12177 binding sites exist in rat interscapular brown adipose tissue (IBAT) plasma membranes. The majority of binding sites are of low affinity with a Kd of 31 nM, a value in close agreement with that for the Kd of [3H]CGP 12177 binding to a cloned rat beta 3-adrenergic receptor (AR) expressed in CHO cells (44 nM). Competition binding studies demonstrate that the Ki values of the cloned rat beta 3-AR and of the low affinity sites in IBAT are 45 and 29 nM, respectively, for BRL 37344 and 1.4 and 1.0 microM, for (-)-propranolol. These findings strongly suggest that the low affinity [3H]CGP 12177 binding site measured in IBAT plasma membranes represents the atypical beta 3-AR in this tissue.Entities:
Mesh：
Substances：
Year: 1992 PMID： 1347503 DOI： 10.1016/0014-5793(92)80046-j
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124