Literature DB >> 1337880

Purification and functional characterization of bovine RP-A in an in vitro SV40 DNA replication system.

H P Nasheuer1, D von Winkler, C Schneider, I Dornreiter, I Gilbert, E Fanning.   

Abstract

The single-stranded DNA binding protein RP-A is required in SV40 DNA in vitro replication. The RP-A purified from calf thymus contains 4 polypeptides with molecular weights 70kDa, 53kDa, 32kDa, and 14kDa. The p70 subunit and its proteolysed form p53 are recognized by the monoclonal antibody 70C (Kenny et al. (1990)) and bind to ssDNA. The p70 and p32 subunits of bovine RP-A are phosphorylated by CDC2-cyclin B kinase. Bovine RP-A supports the origin dependent unwinding of SV40 DNA by T antigen. Furthermore, bovine RP-A can efficiently substitute for human RP-A in SV40 DNA replication in vitro. A modified blotting technique revealed that RP-A interacts specifically and directly with the p48 subunit of DNA polymerase alpha-primase complex.

Entities:  

Mesh：

Substances：

Year:  1992        PMID： 1337880     DOI： 10.1007/bf02451786

Source DB:  PubMed          Journal:  Chromosoma        ISSN： 0009-5915            Impact factor:   4.316


  38 in total

1.  Phosphorylation of large tumour antigen by cdc2 stimulates SV40 DNA replication.

Authors:  D McVey; L Brizuela; I Mohr; D R Marshak; Y Gluzman; D Beach
Journal:  Nature       Date:  1989-10-12       Impact factor: 49.962

2.  Identification of cellular proteins required for simian virus 40 DNA replication.

Authors:  M S Wold; D H Weinberg; D M Virshup; J J Li; T J Kelly
Journal:  J Biol Chem       Date:  1989-02-15       Impact factor: 5.157

Review 3.  SV40 DNA replication.

Authors:  T J Kelly
Journal:  J Biol Chem       Date:  1988-12-05       Impact factor: 5.157

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  DNA polymerase I gene of Saccharomyces cerevisiae: nucleotide sequence, mapping of a temperature-sensitive mutation, and protein homology with other DNA polymerases.

Authors:  A Pizzagalli; P Valsasnini; P Plevani; G Lucchini
Journal:  Proc Natl Acad Sci U S A       Date:  1988-06       Impact factor: 11.205

6.  Influence of poly(ADP-ribose) polymerase on the enzymatic synthesis of SV40 DNA.

Authors:  T Eki; J Hurwitz
Journal:  J Biol Chem       Date:  1991-02-15       Impact factor: 5.157

7.  The role of human single-stranded DNA binding protein and its individual subunits in simian virus 40 DNA replication.

Authors:  M K Kenny; U Schlegel; H Furneaux; J Hurwitz
Journal:  J Biol Chem       Date:  1990-05-05       Impact factor: 5.157

8.  Cell cycle-dependent phosphorylation of human DNA polymerase alpha.

Authors:  H P Nasheuer; A Moore; A F Wahl; T S Wang
Journal:  J Biol Chem       Date:  1991-04-25       Impact factor: 5.157

9.  An essential Saccharomyces cerevisiae single-stranded DNA binding protein is homologous to the large subunit of human RP-A.

Authors:  W D Heyer; M R Rao; L F Erdile; T J Kelly; R D Kolodner
Journal:  EMBO J       Date:  1990-07       Impact factor: 11.598

10.  Cell cycle regulated phosphorylation of RPA-32 occurs within the replication initiation complex.

Authors:  R Fotedar; J M Roberts
Journal:  EMBO J       Date:  1992-06       Impact factor: 11.598
View more
  23 in total

1.  Role of the p68 subunit of human DNA polymerase alpha-primase in simian virus 40 DNA replication.

Authors:  Robert D Ott; Christoph Rehfuess; Vladimir N Podust; Jill E Clark; Ellen Fanning
Journal:  Mol Cell Biol       Date:  2002-08       Impact factor: 4.272

2.  Assembly of the replication initiation complex on SV40 origin DNA.

Authors:  Daniel T Simmons; Dahai Gai; Rebekah Parsons; Amanda Debes; Rupa Roy
Journal:  Nucleic Acids Res       Date:  2004-02-11       Impact factor: 16.971

3.  A cell-free replication system for human polyomavirus JC DNA.

Authors:  J Nesper; R W Smith; A R Kautz; E Sock; M Wegner; F Grummt; H P Nasheuer
Journal:  J Virol       Date:  1997-10       Impact factor: 5.103

4.  Different regions of primase subunit p48 control mouse polyomavirus and simian virus 40 DNA replication in vitro.

Authors:  A R Kautz; A Schneider; K Weisshart; C Geiger; H P Nasheuer
Journal:  J Virol       Date:  2001-02       Impact factor: 5.103

5.  Alkoxylalkyl Esters of Nucleotide Analogs Inhibit Polyomavirus DNA Replication and Large T Antigen Activities.

Authors:  Nichodemus O Onwubiko; Suraya A Diaz; Marcela Krečmerová; Heinz Peter Nasheuer
Journal:  Antimicrob Agents Chemother       Date:  2021-02-17       Impact factor: 5.191

6.  Host-specific replication of BK virus DNA in mouse cell extracts is independently controlled by DNA polymerase alpha-primase and inhibitory activities.

Authors:  Irina Tikhanovich; Heinz Peter Nasheuer
Journal:  J Virol       Date:  2010-04-14       Impact factor: 5.103

7.  Restriction of human polyomavirus BK virus DNA replication in murine cells and extracts.

Authors:  Cathal Mahon; Bo Liang; Irina Tikhanovich; Johanna R Abend; Michael J Imperiale; Heinz P Nasheuer; William R Folk
Journal:  J Virol       Date:  2009-03-18       Impact factor: 5.103

8.  Species-specific functional interactions of DNA polymerase alpha-primase with simian virus 40 (SV40) T antigen require SV40 origin DNA.

Authors:  C Schneider; K Weisshart; L A Guarino; I Dornreiter; E Fanning
Journal:  Mol Cell Biol       Date:  1994-05       Impact factor: 4.272

9.  Species-specific replication of simian virus 40 DNA in vitro requires the p180 subunit of human DNA polymerase alpha-primase.

Authors:  F Stadlbauer; C Voitenleitner; A Brückner; E Fanning; H P Nasheuer
Journal:  Mol Cell Biol       Date:  1996-01       Impact factor: 4.272

10.  Ionizing radiation-dependent and independent phosphorylation of the 32-kDa subunit of replication protein A during mitosis.

Authors:  Holger Stephan; Claire Concannon; Elisabeth Kremmer; Michael P Carty; Heinz-Peter Nasheuer
Journal:  Nucleic Acids Res       Date:  2009-08-11       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.