| Literature DB >> 1333473 |
E S Tackaberry1, J Hamel, Y Larose, R Kuhl, B R Brodeur.
Abstract
A novel enzyme-linked immunosorbent assay (ELISA) was developed for human cytomegalovirus (HCMV) utilizing a monoclonal anti-idiotype specific for CMVB1, an antibody to HCMV. Samples of HCMV were measured by their inhibition of the binding of CMVB1 to anti-idiotype. The ELISA detected HCMV in a concentration-dependent manner from 20 to 0.6 x 10(3) PFU/ml, with 50% inhibition at approx. 3 x 10(3) PFU/ml. These data demonstrate the potential of anti-idiotype antibodies as the basis of simple and rapid diagnostic tests for infectious agents.Entities:
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Year: 1992 PMID： 1333473 DOI： 10.1016/0166-0934(92)90066-m
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014