| Literature DB >> 1328405 |
J T Wang1, J C Sheu, J T Lin, T H Wang, D S Chen.
Abstract
Paired samples of plasma and peripheral blood mononuclear cells (PBMC) from 7 patients with posttransfusion hepatitis C were collected and studied for the presence of replicative forms of hepatitis C virus (HCV). RNA was extracted and tested for both positive and negative strands of HCV RNA by polymerase chain reaction. Positive-strand RNA of HCV was found in both plasma and PBMC of all 7 patients. However, negative-strand RNA was found in PBMC of 3 patients while none was found in the plasma. Levels of negative-strand RNA were about 10-100 times less than those of positive-strand RNA by semiquantification with serial dilution of viral cDNA. The results suggest that active infection and replication of HCV in PBMC is present in patients with posttransfusion hepatitis C and implicate the extrahepatic infection of HCV.Entities:
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Year: 1992 PMID： 1328405 DOI： 10.1093/infdis/166.5.1167
Source DB: PubMed Journal: J Infect Dis ISSN： 0022-1899 Impact factor: 5.226