Literature DB >> 1324737

Direct expression of active spinach glycolate oxidase in Escherichia coli.

P Macheroux1, S B Mulrooney, C H Williams, V Massey.   

Abstract

Spinach glycolate oxidase (GAO) was expressed in Escherichia coli using the T7 RNA polymerase promotor. The enzyme accounts for approx. 1% of the soluble protein fraction and is expressed as a soluble and active enzyme. Comparison with GAO expressed in Saccharomyces cerevisiae (Macheroux, P., Massey, V., Thiele, D.J. and Volokita, M. (1991) Biochemistry 30, 4612-4619) showed that the GAO expressed in E. coli has identical physico-chemical features to the wild-type enzyme, but is expressed at a level approx. 15-fold higher than in the yeast system.

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Year:  1992        PMID： 1324737     DOI： 10.1016/0167-4781(92)90046-3

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  11 in total

1.  Three-dimensional structures of glycolate oxidase with bound active-site inhibitors.

Authors:  K Stenberg; Y Lindqvist
Journal:  Protein Sci       Date:  1997-05       Impact factor: 6.725

2.  Evolution of enzymes involved in the photorespiratory 2-phosphoglycolate cycle from cyanobacteria via algae toward plants.

Authors:  Ramona Kern; Hermann Bauwe; Martin Hagemann
Journal:  Photosynth Res       Date:  2011-01-11       Impact factor: 3.573

3.  The 2.1 A structure of Aerococcus viridans L-lactate oxidase (LOX).

Authors:  Ingar Leiros; Ellen Wang; Tonni Rasmussen; Esko Oksanen; Heidi Repo; Steffen B Petersen; Pirkko Heikinheimo; Edward Hough
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-11-04

4.  Cloning and coexpression of recombinant N-demethylase B and Glycolate oxidase genes in Escherichia coli.

Authors:  Dengchao Li; Qiumin Han; Tong Zhang
Journal:  Mol Biol Rep       Date:  2018-11-29       Impact factor: 2.316

5.  Cyanobacterial lactate oxidases serve as essential partners in N2 fixation and evolved into photorespiratory glycolate oxidases in plants.

Authors:  Claudia Hackenberg; Ramona Kern; Jan Hüge; Lucas J Stal; Yoshinori Tsuji; Joachim Kopka; Yoshihiro Shiraiwa; Hermann Bauwe; Martin Hagemann
Journal:  Plant Cell       Date:  2011-08-09       Impact factor: 11.277

6.  Structure of human glycolate oxidase in complex with the inhibitor 4-carboxy-5-[(4-chlorophenyl)sulfanyl]-1,2,3-thiadiazole.

Authors:  Jean Marie Bourhis; Caroline Vignaud; Nicolas Pietrancosta; Françoise Guéritte; Daniel Guénard; Florence Lederer; Ylva Lindqvist
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2009-11-27

7.  The expression of spinach glycolate oxidase (GO) in E. coli and the application of GO in the production of glyoxylic acid.

Authors:  Jianfeng Jin; Tianwei Tan; Hengliang Wang; Guofu Su
Journal:  Mol Biotechnol       Date:  2003-11       Impact factor: 2.695

8.  Involvement of ionizable groups in catalysis of human liver glycolate oxidase.

Authors:  Andrea Pennati; Giovanni Gadda
Journal:  J Biol Chem       Date:  2009-09-16       Impact factor: 5.157

9.  MftD Catalyzes the Formation of a Biologically Active Redox Center in the Biosynthesis of the Ribosomally Synthesized and Post-translationally Modified Redox Cofactor Mycofactocin.

Authors:  Richard S Ayikpoe; John A Latham
Journal:  J Am Chem Soc       Date:  2019-08-15       Impact factor: 15.419

10.  Solvent and primary deuterium isotope effects show that lactate CH and OH bond cleavages are concerted in Y254F flavocytochrome b2, consistent with a hydride transfer mechanism.

Authors:  Pablo Sobrado; Paul F Fitzpatrick
Journal:  Biochemistry       Date:  2003-12-30       Impact factor: 3.162
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