Literature DB >> 1324186

DNA unwinding activity of replication protein A.

A Georgaki1, B Strack, V Podust, U Hübscher.   

Abstract

Replication protein A (RP-A) is a heterotrimeric complex conserved in eukaryotic cells. It binds to single-stranded DNA and is essential for initiation and elongation of DNA replication. In this communication we give evidence that this protein can unwind DNA independent of magnesium and ATP, two essential cofactors for bona fide DNA helicase activity. RP-A can unwind up to at least 350 basepairs and appears to be required in stoichiometric amounts. The reaction is extremely sensitive to NaCl and MgCl2. This activity of RF-A is suggestive for a possible unwinding function in initiation of DNA replication in eukaryotes.

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Year:  1992        PMID： 1324186     DOI： 10.1016/0014-5793(92)81283-r

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  36 in total

1.  Conserved helicase domain of human RecQ4 is required for strand annealing-independent DNA unwinding.

Authors:  Marie L Rossi; Avik K Ghosh; Tomasz Kulikowicz; Deborah L Croteau; Vilhelm A Bohr
Journal:  DNA Repair (Amst)       Date:  2010-05-06

2.  UV-induced hyperphosphorylation of replication protein a depends on DNA replication and expression of ATM protein.

Authors:  G G Oakley; L I Loberg; J Yao; M A Risinger; R L Yunker; M Zernik-Kobak; K K Khanna; M F Lavin; M P Carty; K Dixon
Journal:  Mol Biol Cell       Date:  2001-05       Impact factor: 4.138

3.  Multimerization of the adenovirus DNA-binding protein is the driving force for ATP-independent DNA unwinding during strand displacement synthesis.

Authors:  J Dekker; P N Kanellopoulos; A K Loonstra; J A van Oosterhout; K Leonard; P A Tucker; P C van der Vliet
Journal:  EMBO J       Date:  1997-03-17       Impact factor: 11.598

4.  Double-check probing of DNA bending and unwinding by XPA-RPA: an architectural function in DNA repair.

Authors:  M Missura; T Buterin; R Hindges; U Hübscher; J Kaspárková; V Brabec; H Naegeli
Journal:  EMBO J       Date:  2001-07-02       Impact factor: 11.598

5.  Annealing helicase 2 (AH2), a DNA-rewinding motor with an HNH motif.

Authors:  Timur Yusufzai; James T Kadonaga
Journal:  Proc Natl Acad Sci U S A       Date:  2010-11-15       Impact factor: 11.205

6.  Photocrosslinking locates a binding site for the large subunit of human replication protein A to the damaged strand of cisplatin-modified DNA.

Authors:  U Schweizer; T Hey; G Lipps; G Krauss
Journal:  Nucleic Acids Res       Date:  1999-08-01       Impact factor: 16.971

7.  Mechanism of open complex and dual incision formation by human nucleotide excision repair factors.

Authors:  E Evans; J G Moggs; J R Hwang; J M Egly; R D Wood
Journal:  EMBO J       Date:  1997-11-03       Impact factor: 11.598

8.  DNA-binding polarity of human replication protein A positions nucleases in nucleotide excision repair.

Authors:  W L de Laat; E Appeldoorn; K Sugasawa; E Weterings; N G Jaspers; J H Hoeijmakers
Journal:  Genes Dev       Date:  1998-08-15       Impact factor: 11.361

9.  Torsional regulation of hRPA-induced unwinding of double-stranded DNA.

Authors:  Iwijn De Vlaminck; Iztok Vidic; Marijn T J van Loenhout; Roland Kanaar; Joyce H G Lebbink; Cees Dekker
Journal:  Nucleic Acids Res       Date:  2010-03-02       Impact factor: 16.971

10.  Purification and characterisation of a DNA helicase, dheI I, from Drosophila melanogaster embryos.

Authors:  P Thömmes; R F Marton; S Cotterill
Journal:  Nucleic Acids Res       Date:  1995-11-11       Impact factor: 16.971
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