Literature DB >> 1322933

Comparison of several PCR procedures for detection of serum HCV-RNA using different regions of the HCV genome.

I Castillo1, J Bartolomé, J A Quiroga, V Carreño.   

Abstract

Different methods for the isolation of hepatitis C virus RNA and several sets of primers from the 5' untranslated, core, NS3, NS3/NS4 and NS5 regions of the hepatitis C virus genome were used for the detection of the viral genome by the polymerase chain reaction. Serum samples from 10 patients with chronic hepatitis C and 10 healthy controls were studied. The best method for the RNA extraction was with cold guanidinium isothiocyanate followed by a denaturation step prior to the polymerase chain reaction. Using this method, the percentage of positivity to hepatitis C virus sequences in serum depending on the region amplified were: 5' untranslated, 90%; Core, 20%; NS3, 80%; NS3/NS4, 60% and NS5, 60%.

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Year:  1992        PMID： 1322933     DOI： 10.1016/0166-0934(92)90170-i

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  5 in total

1.  Concordance of hepatitis C virus typing methods based on restriction fragment length polymorphism analysis in 5' noncoding region and NS4 serotyping, but not in core PCR or a line probe assay.

Authors:  S Navas; I Castillo; J Martín; J A Quiroga; J Bartolomé; V Carreño
Journal:  J Clin Microbiol       Date:  1997-01       Impact factor: 5.948

2.  Genomic and phylogenetic analysis of hepatitis C virus isolates from argentine patients: a six-year retrospective study.

Authors:  J F Quarleri; B H Robertson; V L Mathet; M Feld; L Espínola; M P Requeijo; O Mandó; G Carballal; J R Oubiña
Journal:  J Clin Microbiol       Date:  2000-12       Impact factor: 5.948

3.  In situ detection of hepatitis C virus RNA in salivary glands.

Authors:  J J Arrieta; E Rodríguez-Iñigo; N Ortiz-Movilla; J Bartolomé; M Pardo; F Manzarbeitia; H Oliva; D M Macías; V Carreño
Journal:  Am J Pathol       Date:  2001-01       Impact factor: 4.307

4.  Evidence of subtype-specific antibodies to antigenic epitopes in the NS5 region of hepatitis C virus in the circulation of patients with chronic hepatitis C.

Authors:  J A Quiroga; J Martin; M Pernas; M Pardo; M Herrero; I Castillo; J Bartolome; V Carreño
Journal:  Clin Diagn Lab Immunol       Date:  1994-09

5.  Multigene tracking of hepatitis C virus quasispecies after liver transplantation: correlation of genetic diversification in the envelope region with asymptomatic or mild disease patterns.

Authors:  D G Sullivan; J J Wilson; R L Carithers; J D Perkins; D R Gretch
Journal:  J Virol       Date:  1998-12       Impact factor: 5.103
  5 in total

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