Literature DB >> 1322797

Phosphatidylinositol 3-kinase: structure and expression of the 110 kd catalytic subunit.

I D Hiles1, M Otsu, S Volinia, M J Fry, I Gout, R Dhand, G Panayotou, F Ruiz-Larrea, A Thompson, N F Totty.   

Abstract

Purified bovine brain phosphatidylinositol 3-kinase (Pl3-kinase) is composed of 85 kd and 110 kd subunits. The 85 kd subunit (p85 alpha) lacks Pl3-kinase activity and acts as an adaptor, coupling the 110 kd subunit (p110) to activated protein tyrosine kinases. Here the characterization of the p110 subunit is presented. cDNA cloning reveals p110 to be a 1068 aa protein related to Vps34p, a S. cerevisiae protein involved in the sorting of proteins to the vacuole. p110 expressed in insect cells possesses Pl3-kinase activity and associates with p85 alpha into an active p85 alpha-p110 complex that binds the activated colony-stimulating factor 1 receptor. p110 expressed in COS-1 cells is catalytically active only when complexed with p85 alpha.

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Year:  1992        PMID： 1322797     DOI： 10.1016/0092-8674(92)90166-a

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  183 in total

1.  Regulation of nuclear translocation of forkhead transcription factor AFX by protein kinase B.

Authors:  H Takaishi; H Konishi; H Matsuzaki; Y Ono; Y Shirai; N Saito; T Kitamura; W Ogawa; M Kasuga; U Kikkawa; Y Nishizuka
Journal:  Proc Natl Acad Sci U S A       Date:  1999-10-12       Impact factor: 11.205

2.  Comparison of the kinetic properties of the lipid- and protein-kinase activities of the p110alpha and p110beta catalytic subunits of class-Ia phosphoinositide 3-kinases.

Authors:  C A Beeton; E M Chance; L C Foukas; P R Shepherd
Journal:  Biochem J       Date:  2000-09-01       Impact factor: 3.857

3.  The p85 subunit of phosphoinositide 3-kinase is associated with beta-catenin in the cadherin-based adhesion complex.

Authors:  R J Woodfield; M N Hodgkin; N Akhtar; M A Morse; K J Fuller; K Saqib; N T Thompson; M J Wakelam
Journal:  Biochem J       Date:  2001-12-01       Impact factor: 3.857

Review 4.  Phosphatidylinositol 3' kinase signaling in mammary tumorigenesis.

Authors:  M P Scheid; J R Woodgett
Journal:  J Mammary Gland Biol Neoplasia       Date:  2001-01       Impact factor: 2.673

5.  Mechanism of the regulation of type IB phosphoinositide 3OH-kinase byG-protein betagamma subunits.

Authors:  Sonja Krugmann; Matthew A Cooper; Dudley H Williams; Phillip T Hawkins; Len R Stephens
Journal:  Biochem J       Date:  2002-03-15       Impact factor: 3.857

6.  Inducible gene expression and protein translocation using nontoxic ligands identified by a mammalian three-hybrid screen.

Authors:  S D Liberles; S T Diver; D J Austin; S L Schreiber
Journal:  Proc Natl Acad Sci U S A       Date:  1997-07-22       Impact factor: 11.205

7.  Association of the insulin receptor and phosphatidylinositol 3-kinase requires a third component.

Authors:  R Liu; J N Livingston
Journal:  Biochem J       Date:  1994-01-15       Impact factor: 3.857

8.  Identification of a proline-rich sequence in the CD2 cytoplasmic domain critical for regulation of integrin-mediated adhesion and activation of phosphoinositide 3-kinase.

Authors:  W J Kivens; S W Hunt; J L Mobley; T Zell; C L Dell; B E Bierer; Y Shimizu
Journal:  Mol Cell Biol       Date:  1998-09       Impact factor: 4.272

9.  The inability of phosphatidylinositol 3-kinase activation to stimulate GLUT4 translocation indicates additional signaling pathways are required for insulin-stimulated glucose uptake.

Authors:  S J Isakoff; C Taha; E Rose; J Marcusohn; A Klip; E Y Skolnik
Journal:  Proc Natl Acad Sci U S A       Date:  1995-10-24       Impact factor: 11.205

10.  The v-Src SH3 domain binds phosphatidylinositol 3'-kinase.

Authors:  X Liu; L E Marengere; C A Koch; T Pawson
Journal:  Mol Cell Biol       Date:  1993-09       Impact factor: 4.272
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