OBJECTIVE: To evaluate the effects of intraarticular injection of recombinant human interleukin-1 beta (IL-1 beta) on levels of proteoglycans, stromelysin, and leukocytes in rabbit synovial fluid (SF), and to determine the effects of leukocyte depletion on SF proteoglycan and stromelysin levels. METHODS: Levels of leukocytes and of proteoglycans, stromelysin, and collagenase were evaluated 12 hours after the intraarticular injection of various doses of IL-1, and over a 24-hour period after injection at a single dose level. We used a monoclonal antibody (MAb) against leukocyte integrins, which markedly depressed leukocyte accumulation in SF, to evaluate the role of synovial leukocytes on IL-1-induced increases in SF proteoglycan and stromelysin levels. RESULTS: Levels of both proteoglycans and stromelysin increased in the IL-1-injected joints between 4 hours and 24 hours after the injection of a single 200-ng dose of IL-1. The highest levels of stromelysin and proteoglycans were achieved with IL-1 doses greater than or equal to 100 ng. Infiltration of polymorphonuclear cells (PMN) into the joint fluid of the IL-1-injected rabbits also increased, in a dose-dependent manner. Treatment of rabbits with MAb 1B4 markedly reduced infiltration of PMN into the joint, without affecting either stromelysin or proteoglycan levels. CONCLUSION: Taken together, the data suggest that there is a coordinate increase in SF stromelysin and proteoglycan levels in rabbits injected with IL-1, and that leukocytes play a minimal role in the accumulation of proteoglycans and stromelysin in the SF.
OBJECTIVE: To evaluate the effects of intraarticular injection of recombinant humaninterleukin-1 beta (IL-1 beta) on levels of proteoglycans, stromelysin, and leukocytes in rabbit synovial fluid (SF), and to determine the effects of leukocyte depletion on SF proteoglycan and stromelysin levels. METHODS: Levels of leukocytes and of proteoglycans, stromelysin, and collagenase were evaluated 12 hours after the intraarticular injection of various doses of IL-1, and over a 24-hour period after injection at a single dose level. We used a monoclonal antibody (MAb) against leukocyte integrins, which markedly depressed leukocyte accumulation in SF, to evaluate the role of synovial leukocytes on IL-1-induced increases in SF proteoglycan and stromelysin levels. RESULTS: Levels of both proteoglycans and stromelysin increased in the IL-1-injected joints between 4 hours and 24 hours after the injection of a single 200-ng dose of IL-1. The highest levels of stromelysin and proteoglycans were achieved with IL-1 doses greater than or equal to 100 ng. Infiltration of polymorphonuclear cells (PMN) into the joint fluid of the IL-1-injected rabbits also increased, in a dose-dependent manner. Treatment of rabbits with MAb 1B4 markedly reduced infiltration of PMN into the joint, without affecting either stromelysin or proteoglycan levels. CONCLUSION: Taken together, the data suggest that there is a coordinate increase in SF stromelysin and proteoglycan levels in rabbits injected with IL-1, and that leukocytes play a minimal role in the accumulation of proteoglycans and stromelysin in the SF.
Authors: M W Lark; H Williams; L A Hoernner; J Weidner; J M Ayala; C F Harper; A Christen; J Olszewski; Z Konteatis; R Webber Journal: Biochem J Date: 1995-04-01 Impact factor: 3.857
Authors: I I Singer; D W Kawka; E K Bayne; S A Donatelli; J R Weidner; H R Williams; J M Ayala; R A Mumford; M W Lark; T T Glant Journal: J Clin Invest Date: 1995-05 Impact factor: 14.808
Authors: G Bandara; G M Mueller; J Galea-Lauri; M H Tindal; H I Georgescu; M K Suchanek; G L Hung; J C Glorioso; P D Robbins; C H Evans Journal: Proc Natl Acad Sci U S A Date: 1993-11-15 Impact factor: 11.205
Authors: V Knäuper; S M Wilhelm; P K Seperack; Y A DeClerck; K E Langley; A Osthues; H Tschesche Journal: Biochem J Date: 1993-10-15 Impact factor: 3.857
Authors: G Schett; K Redlich; Q Xu; P Bizan; M Gröger; M Tohidast-Akrad; H Kiener; J Smolen; G Steiner Journal: J Clin Invest Date: 1998-07-15 Impact factor: 14.808