RATIONALE AND OBJECTIVES: The detection of arthritis by autofluorescence was investigated using an antigen-induced arthritis model. METHODS: For autofluorescence investigations of joints, a mobile fluorescence-detector was constructed consisting of a lens/mirror system attached to a conventional spectrofluorometer and optimized fiber optic cables reaching to and from the site of investigation. Autofluorescence measurements were performed at 7 arthritic and 7 healthy mice. Fifteen antigen-induced arthritis and 3 healthy mice were used for histologic examinations. RESULTS: In the exudative stage (day 1), a decrease of emission signal intensities for excitation wavelengths at 300 nm (emission, 355-365 nm) and 360 nm (emission, 475-485 nm) was observed. Signals increased on day 7 (maximum of cellular infiltration). Chronic inflammation (day 14 and 21) led to a decrease of signals again. CONCLUSION: Arthritis influences autofluorescence signals in vivo. The detected excitation/emission pairs can be assigned to collagen/elastin and NAD(P)H. Signal intensities of NAD(P)H differed significantly from controls at day 1 and 7.
RATIONALE AND OBJECTIVES: The detection of arthritis by autofluorescence was investigated using an antigen-induced arthritis model. METHODS: For autofluorescence investigations of joints, a mobile fluorescence-detector was constructed consisting of a lens/mirror system attached to a conventional spectrofluorometer and optimized fiber optic cables reaching to and from the site of investigation. Autofluorescence measurements were performed at 7 arthritic and 7 healthy mice. Fifteen antigen-induced arthritis and 3 healthy mice were used for histologic examinations. RESULTS: In the exudative stage (day 1), a decrease of emission signal intensities for excitation wavelengths at 300 nm (emission, 355-365 nm) and 360 nm (emission, 475-485 nm) was observed. Signals increased on day 7 (maximum of cellular infiltration). Chronic inflammation (day 14 and 21) led to a decrease of signals again. CONCLUSION:Arthritis influences autofluorescence signals in vivo. The detected excitation/emission pairs can be assigned to collagen/elastin and NAD(P)H. Signal intensities of NAD(P)H differed significantly from controls at day 1 and 7.
Authors: Alexander Pfeil; Joachim Böttcher; Bettina E Seidl; Jens-Peter Heyne; Alexander Petrovitch; Torsten Eidner; Hans-Joachim Mentzel; Gunter Wolf; Gert Hein; Werner A Kaiser Journal: Skeletal Radiol Date: 2007-05-17 Impact factor: 2.199
Authors: Alexander Pfeil; Karl F Drummer; Joachim Böttcher; Christian Jung; Peter Oelzner; Diane M Renz; Marcus Franz; Andreas Hansch; Gunter Wolf Journal: Biomed Res Int Date: 2015-08-18 Impact factor: 3.411