| Literature DB >> 12950388 |
H-M Oh1, J-M Oh, S-C Choi, S-W Kim, W-C Han, T-H Kim, D-S Park, C-D Jun.
Abstract
Several methods have been developed for the immortalization of B lymphocytes by Epstein-Barr virus (EBV). We developed an efficient method which reduces the time from culture initiation to immortalization and cryopreservation. Two infections of EBV to lymphocytes, and the use of phorbol ester-induced EBV stock significantly improved immortalization efficiency and reduced the time between initiation and immortalization and cryopreservation. The resulting cell bank was used to produce DNA for genetic studies focusing on the genes involved in immune and autistic disorders.Entities:
Mesh：
Year: 2003 PMID： 12950388 PMCID： PMC6495897 DOI： 10.1046/j.1365-2184.2003.00276.x
Source DB: PubMed Journal: Cell Prolif ISSN： 0960-7722 Impact factor: 6.831