Literature DB >> 7735567

An efficient method for routine Epstein-Barr virus immortalization of human B lymphocytes.

F E Wall1, R D Henkel, M P Stern, H B Jenson, M P Moyer.   

Abstract

A variety of methods exist for the immortalization of B lymphocytes by Epstein-Barr virus due to the simplicity of such techniques to establish cell lines with stable genomic DNA. Two different methods for immortalizing lymphoblastoid cell lines were compared for differences in techniques and materials, time between initiation and immortalization, and success rate of immortalization. An incubation period in Epstein-Barr virus and the use of conditioned media improved immortalization efficiency from 86 to 98% and decreased the time (usually weeks) from culture initiation to cryopreservation. The resulting cell bank was used to produce DNA for genetic studies focusing on the genes involved in non-insulin-dependent diabetes mellitus.

Entities:  

Mesh：

Substances：

Year:  1995        PMID： 7735567     DOI： 10.1007/BF02633976

Source DB:  PubMed          Journal:  In Vitro Cell Dev Biol Anim        ISSN： 1071-2690            Impact factor:   2.416


  34 in total

1.  Sample age and Epstein-Barr virus transformation of cryopreserved lymphocytes.

Authors:  J A Reidy; V A Wheeler
Journal:  In Vitro Cell Dev Biol       Date:  1992-06

Review 2.  Immortalizing genes of Epstein-Barr virus.

Authors:  T Middleton; T A Gahn; J M Martin; B Sugden
Journal:  Adv Virus Res       Date:  1991       Impact factor: 9.937

3.  The growth transformation of human B cells involves superinduction of hsp70 and hsp90.

Authors:  R K Cheung; H M Dosch
Journal:  Virology       Date:  1993-04       Impact factor: 3.616

4.  Activation and immortalization of leukaemic B cells by Epstein-Barr virus.

Authors:  E V Walls; M G Doyle; K K Patel; M J Allday; D Catovsky; D H Crawford
Journal:  Int J Cancer       Date:  1989-11-15       Impact factor: 7.396

5.  Establishment of continuous lymphoblast cultures from leukocytes of gibbons (Hylobates lar).

Authors:  J Werner; G Henle; C A Pinto; R F Haff; W Henle
Journal:  Int J Cancer       Date:  1972-11       Impact factor: 7.396

6.  Separation of leukocytes from blood and bone marrow. Introduction.

Authors:  A Böyum
Journal:  Scand J Clin Lab Invest Suppl       Date:  1968

7.  Epstein-Barr virus-induced transformation of human leukocytes after cell fractionation.

Authors:  U Schneider; H zur Hausen
Journal:  Int J Cancer       Date:  1975-01-15       Impact factor: 7.396

8.  Human B lymphocytes immortalization by Epstein-Barr virus in the presence of cyclosporin A.

Authors:  F Pelloquin; J P Lamelin; G M Lenoir
Journal:  In Vitro Cell Dev Biol       Date:  1986-12

9.  Transition of phenotypic dimorphism with regard to spontaneous sister chromatid exchange in Epstein-Barr virus-transformed Bloom's syndrome lymphoblastoid cell lines.

Authors:  K Tatsumi; T Kurihara; I Arita; J Tatsumi-Miyajima
Journal:  Jpn J Cancer Res       Date:  1992-07

10.  Characteristics of Epstein-Barr virus activation of human B lymphocytes.

Authors:  A G Bird; S Britton; I Ernberg; K Nilsson
Journal:  J Exp Med       Date:  1981-09-01       Impact factor: 14.307
View more
  18 in total

1.  Transduction of human NOD/SCID-repopulating cells with both lymphoid and myeloid potential by foamy virus vectors.

Authors:  Neil C Josephson; George Vassilopoulos; Grant D Trobridge; Greg V Priestley; Brent L Wood; Thalia Papayannopoulou; David W Russell
Journal:  Proc Natl Acad Sci U S A       Date:  2002-06-11       Impact factor: 11.205

2.  Do US Black Women Experience Stress-Related Accelerated Biological Aging?: A Novel Theory and First Population-Based Test of Black-White Differences in Telomere Length.

Authors:  Arline T Geronimus; Margaret T Hicken; Jay A Pearson; Sarah J Seashols; Kelly L Brown; Tracey Dawson Cruz
Journal:  Hum Nat       Date:  2010-03-10

3.  Comparison of 12 reference genes for normalization of gene expression levels in Epstein-Barr virus-transformed lymphoblastoid cell lines and fibroblasts.

Authors:  Arjan P M de Brouwer; Hans van Bokhoven; Hannie Kremer
Journal:  Mol Diagn Ther       Date:  2006       Impact factor: 4.074

4.  TDP2 protects transcription from abortive topoisomerase activity and is required for normal neural function.

Authors:  Fernando Gómez-Herreros; Janneke H M Schuurs-Hoeijmakers; Mark McCormack; Marie T Greally; Stuart Rulten; Rocío Romero-Granados; Timothy J Counihan; Elijah Chaila; Judith Conroy; Sean Ennis; Norman Delanty; Felipe Cortés-Ledesma; Arjan P M de Brouwer; Gianpiero L Cavalleri; Sherif F El-Khamisy; Bert B A de Vries; Keith W Caldecott
Journal:  Nat Genet       Date:  2014-03-23       Impact factor: 38.330

5.  Disruption of intraflagellar protein transport in photoreceptor cilia causes Leber congenital amaurosis in humans and mice.

Authors:  Karsten Boldt; Dorus A Mans; Jungyeon Won; Jeroen van Reeuwijk; Andreas Vogt; Norbert Kinkl; Stef J F Letteboer; Wanda L Hicks; Ron E Hurd; Jürgen K Naggert; Yves Texier; Anneke I den Hollander; Robert K Koenekoop; Jean Bennett; Frans P M Cremers; Christian J Gloeckner; Patsy M Nishina; Ronald Roepman; Marius Ueffing
Journal:  J Clin Invest       Date:  2011-05-23       Impact factor: 14.808

6.  A 3-base pair deletion, c.9711_9713del, in DMD results in intellectual disability without muscular dystrophy.

Authors:  Arjan P M de Brouwer; Sander B Nabuurs; Ingrid E C Verhaart; Astrid R Oudakker; Roel Hordijk; Helger G Yntema; Jannet M Hordijk-Hos; Krysta Voesenek; Bert B A de Vries; Ton van Essen; Wei Chen; Hao Hu; Jamel Chelly; Johan T den Dunnen; Vera M Kalscheuer; Annemieke M Aartsma-Rus; Ben C J Hamel; Hans van Bokhoven; Tjitske Kleefstra
Journal:  Eur J Hum Genet       Date:  2013-07-31       Impact factor: 4.246

7.  CDK19 is disrupted in a female patient with bilateral congenital retinal folds, microcephaly and mild mental retardation.

Authors:  Arijit Mukhopadhyay; Jamie M Kramer; Gerard Merkx; Dorien Lugtenberg; Dominique F Smeets; Merel A W Oortveld; Ellen A W Blokland; Jyoti Agrawal; Annette Schenck; Hans van Bokhoven; Erik Huys; Eric F Schoenmakers; Ad Geurts van Kessel; C Erik van Nouhuys; Frans P M Cremers
Journal:  Hum Genet       Date:  2010-06-22       Impact factor: 4.132

8.  Arts syndrome is caused by loss-of-function mutations in PRPS1.

Authors:  Arjan P M de Brouwer; Kelly L Williams; John A Duley; André B P van Kuilenburg; Sander B Nabuurs; Michael Egmont-Petersen; Dorien Lugtenberg; Lida Zoetekouw; Martijn J G Banning; Melissa Roeffen; Ben C J Hamel; Linda Weaving; Robert A Ouvrier; Jennifer A Donald; Ron A Wevers; John Christodoulou; Hans van Bokhoven
Journal:  Am J Hum Genet       Date:  2007-08-03       Impact factor: 11.025

9.  OFD1 is mutated in X-linked Joubert syndrome and interacts with LCA5-encoded lebercilin.

Authors:  Karlien L M Coene; Ronald Roepman; Dan Doherty; Bushra Afroze; Hester Y Kroes; Stef J F Letteboer; Lock H Ngu; Bartlomiej Budny; Erwin van Wijk; Nicholas T Gorden; Malika Azhimi; Christel Thauvin-Robinet; Joris A Veltman; Mireille Boink; Tjitske Kleefstra; Frans P M Cremers; Hans van Bokhoven; Arjan P M de Brouwer
Journal:  Am J Hum Genet       Date:  2009-10       Impact factor: 11.025

10.  Spectral properties and mechanisms that underlie autofluorescent accumulations in Batten disease.

Authors:  Sabrina S Seehafer; David A Pearce
Journal:  Biochem Biophys Res Commun       Date:  2009-02-25       Impact factor: 3.575
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.