Literature DB >> 12948134

Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize.

Michal Masarik1, Rene Kizek, Karl J Kramer, Sabina Billova, Marie Brazdova, Jan Vacek, Michele Bailey, Frantisek Jelen, John A Howard.   

Abstract

The proteins streptavidin and avidin were electrochemically detected in solution by adsorptive transfer stripping square wave voltammetry (AdTS SWV) at a carbon paste electrode (CPE). AdTS SWV was used to quantify biotinylated oligonucleotides, DNA hybridizations, and avidin in extracts of transgenic avidin maize. The detection limits of denatured and native streptavidin were 6 pM and 120 nM, respectively. The results demonstrated that streptavidin/avidin AdTS SWV is a sensitive and specific method for quantifying DNA and proteins in biological samples such as foods and tissue extracts, including genetically modified crops (avidin maize) and other plants in neighboring fields.

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Year:  2003        PMID： 12948134     DOI： 10.1021/ac020788z

Source DB:  PubMed          Journal:  Anal Chem        ISSN： 0003-2700            Impact factor:   6.986


  12 in total

1.  Maize-Produced Ag2 as a Subunit Vaccine for Valley Fever.

Authors:  Celine A Hayden; Chiung-Yu Hung; Hao Zhang; Austin Negron; Raymond Esquerra; Gary Ostroff; Ambily Abraham; Alejandro Gabriel Lopez; Juliet Elizabeth Gonzales; John A Howard
Journal:  J Infect Dis       Date:  2019-07-19       Impact factor: 5.226

2.  Multi-instrumental Analysis of Tissues of Sunflower Plants Treated with Silver(I) Ions - Plants as Bioindicators of Environmental Pollution.

Authors:  Sona Krizkova; Pavel Ryant; Olga Krystofova; Vojtech Adam; Michaela Galiova; Miroslava Beklova; Petr Babula; Jozef Kaiser; Karel Novotny; Jan Novotny; Miroslav Liska; Radomir Malina; Josef Zehnalek; Jaromir Hubalek; Ladislav Havel; Rene Kizek
Journal:  Sensors (Basel)       Date:  2008-01-24       Impact factor: 3.576

3.  An Electrochemical Detection of Metallothioneins at the Zeptomole Level in Nanolitre Volumes.

Authors:  Vojtech Adam; Jiri Baloun; Ivo Fabrik; Libuse Trnkova; Rene Kizek
Journal:  Sensors (Basel)       Date:  2008-04-01       Impact factor: 3.576

4.  Change of the protein p53 electrochemical signal according to its structural form - quick and sensitive distinguishing of native, denatured, and aggregated form of the "guardian of the genome".

Authors:  David Potesil; Radka Mikelova; Vojtech Adam; Rene Kizek; Richard Prusa
Journal:  Protein J       Date:  2006-01       Impact factor: 4.000

5.  Silver(I) ions ultrasensitive detection at carbon electrodes-analysis of waters, tobacco cells and fish tissues.

Authors:  Sona Krizkova; Olga Krystofova; Libuse Trnkova; Jaromir Hubalek; Vojtech Adam; Miroslava Beklova; Ales Horna; Ladislav Havel; Rene Kizek
Journal:  Sensors (Basel)       Date:  2009-09-01       Impact factor: 3.576

6.  Carbon composite micro- and nano-tubes-based electrodes for detection of nucleic acids.

Authors:  Jan Prasek; Dalibor Huska; Ondrej Jasek; Lenka Zajickova; Libuse Trnkova; Vojtech Adam; Rene Kizek; Jaromir Hubalek
Journal:  Nanoscale Res Lett       Date:  2011-05-16       Impact factor: 4.703

7.  Amperometric Sensor for Detection of Chloride Ions.

Authors:  Libuse Trnkova; Vojtech Adam; Jaromir Hubalek; Petr Babula; Rene Kizek
Journal:  Sensors (Basel)       Date:  2008-09-15       Impact factor: 3.576

8.  Specific Magnetic Isolation of E6 HPV16 Modified Magnetizable Particles Coupled with PCR and Electrochemical Detection.

Authors:  Ana Maria Jimenez Jimenez; Branislav Ruttkay-Nedecky; Simona Dostalova; Ludmila Krejcova; Petr Michalek; Lukas Richtera; Vojtech Adam
Journal:  Int J Mol Sci       Date:  2016-05-05       Impact factor: 5.923

9.  Utilizing of Square Wave Voltammetry to Detect Flavonoids in the Presence of Human Urine.

Authors:  Vojtech Adam; Radka Mikelova; Jaromír Hubalek; Pavel Hanustiak; Miroslava Beklova; Petr Hodek; Ales Horna; Libuse Trnkova; Marie Stiborova; Ladislav Zeman; Rene Kizek
Journal:  Sensors (Basel)       Date:  2007-10-19       Impact factor: 3.576

10.  Direct in vivo electrochemical detection of haemoglobin in red blood cells.

Authors:  Rou Jun Toh; Weng Kung Peng; Jongyoon Han; Martin Pumera
Journal:  Sci Rep       Date:  2014-08-28       Impact factor: 4.379
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