Are smoking and chlamydial infection risk factors for CIN? Different results after adjustment for HPV DNA and antibodies.

To identify the risk factors for cervical intraepithelial neoplasia (CIN), we reanalysed the data from our previous case-control study by adjusting for human papillomavirus (HPV) antibodies. Unlike our previous study based only on HPV DNA, smoking and Chlamydia trachomatis infection were revealed as significant risk factors for CIN after adjustment for HPV antibodies.

Infections by oncogenic human papillomaviruses (HPVs) are established as a major risk factor for cervical intraepithelial neoplasia (CIN) and invasive cervical cancer (ICC). However, only a fraction of infected women develop cervical cancer, suggesting the involvement of additional cofactors in cervical carcinogenesis. In numerous case–control studies various epidemiological factors have been suggested as relevant to CIN and ICC, although the results from these studies have not been entirely consistent (Schiffman and Brinton, 1995). In epidemiological studies of risk factors for CIN and ICC, it is of great importance to allow for the strong effect of HPV infections. To determine HPV exposure among cases and controls, most studies have used HPV DNA testing, while recent studies have employed HPV capsid serology. However, it has not been determined whether the different measurements of HPV exposure can affect conclusions from case–control studies.

To address this issue, we reanalysed the data from our previous case–control study of CIN by adjusting for HPV DNA and antibodies.

MATERIALS AND METHODS

The present study was conducted on women who previously participated in a Japanese case–control study of CIN. The details about this case–control study have been provided elsewhere (Yoshikawa ). Among a total of 167 pairs, serum samples from 26 cases and 58 controls were not available for HPV capsid serology. Therefore, the present analysis was restricted to 250 subjects consisting of 141 cases (80 CIN I, 34 CIN II and 27 CIN III) and 109 controls that were tested for both cervical HPV DNA and serum HPV antibodies. The distributions of study variables in the excluded/included cases and controls were similar. We examined HPV DNA in cervical samples by polymerase chain reaction (PCR) using consensus primers for the HPV L1 region (Yoshikawa ). Detection of IgG antibodies to HPV16, 52 and 58, the most frequently detected HPV types in Japan, was performed by enzyme-linked immunosorbent assay (ELISA) using purified L1-capsids (virus-like particles (VLPs)) as antigens (Matsumoto ). In addition, the level of IgG antibodies to Chlamydia trachomatis was determined by using an enzyme immunoassay (EIA) kit (Thermo Labsystems, Vantaa, Finland) that does not detect antibody to Chlamydia pneumoniae. The adjusted odds ratios (ORs) and 95% confidence intervals (CIs) were estimated by a logistic regression analysis. The analysis was carried out using JMP 4.0J statistics package (SAS Institute, Cary, NC, USA). The P-values obtained in all tests were considered significant at <0.05.

RESULTS

Human papillomavirus status among cases and controls was determined by using HPV DNA testing and HPV capsid serology (Table 1). We reconfirmed that HPV DNA positivity and HPV seropositivity were strongly associated with CIN development (P<0.0001 and =0.0002, respectively), although the relative risk for CIN was considerably different between HPV DNA positive and seropositive women (17.9 and 2.7, respectively) (Table 2). The estimated risks for CIN development in relation to various epidemiological factors are shown in Table 3. In the present study, the adjusted analysis for HPV DNA alone gave the same results as our previous study (Yoshikawa ). Marriage, multiparity and age at first pregnancy were found to be significant risk factors for CIN development in the crude and adjusted analyses. Sexual behaviour, including age at first intercourse and lifetime number of sexual partners, was significantly associated with an increased risk of CIN in the crude analysis, but the significance disappeared in both adjusted analyses. Current smoking and C. trachomatis infection were revealed as significant cofactors for CIN development after adjusting for HPV antibodies, although not after adjusting for HPV DNA. We found no association between the use of oral contraceptives (OC) and CIN development because of the low prevalence of OC users among study subjects. The effect of smoking on CIN development did not appear to vary between HPV-positive and HPV-negative subjects, while the effect of C. trachomatis infection was stronger among the former.

Table 1

Human papillomavirus status among cases and controls

HPV status
DNA	Antibodiesa	Case (n=141)	Control (n=109)
—	—	21 (15%)	65 (60%)
—	+	15 (11%)	28 (26%)
+	—	44 (31%)	11 (10%)
+	+	61 (43%)	5 (4%)

IgG antibodies to any type of HPV16/52/58 L1-capsids.

Table 2

Human papillomavirus infections and the estimated risks for CIN

	Case (n=141)	Control (n=109)	OR (95% CI)a
HPV DNA
Negative	36 (26%)	93 (85%)	1.0
Positive	105 (74%)	16 (15%)	17.9 (9.5–36.0)
HPV antibodiesb
Negative	65 (46%)	76 (70%)	1.0
Positive	76 (54%)	33 (30%)	2.7 (1.6–4.6)

odds ratios and 95% confidence intervals adjusted for age.

IgG antibodies to any type of HPV16/52/58 L1-capsids.

Table 3

Estimated risks for CIN associated with epidemiological cofactors

Variables	Number of cases/controls	Crude ORa (95% CI)b	OR adjusted for HPV DNA and Age (95% CI)	OR adjusted for HPV Abc and Age (95% CI)
Marital status
Never married	8/14	1.0	1.0	1.0
Separated/widowed	12/11	1.9 (0.6–6.3)	0.5 (0.1–2.7)	1.3 (0.3–5.1)
Married	121/84	2.5 (1.0–6.1)	3.9 (1.2–13.3)	2.8 (1.1–7.4)
		P=0.04	P=0.04	P=0.04
Parity
0	12/18	1.0	1.0	1.0
1–2	78/66	1.7 (0.8–3.9)	2.0 (0.7–5.9)	1.3 (0.6–2.9)
3+	50/24	3.1 (1.3–7.4)	4.7 (1.4–15.7)	3.5 (1.4–9.1)
		P for trend=0.01	P=0.01	P=0.03
Age at first pregnancy (years)
–23	63/26	2.0 (1.1–3.7)	3.5 (1.4–9.2)	3.5 (1.6–7.9)
24–26	37/38	1.0	1.0	1.0
27+	31/26	1.1 (0.6–2.1)	0.9 (0.3–2.6)	0.7 (0.3–1.7)
		P for trend=0.03	P=0.01	P=0.01
Cigarette smoking
Never	94/78	1.0	1.0	1.0
Past	13/14	0.8 (0.3–1.7)	0.3 (0.1–1.1)	0.4 (0.1–1.2)
Current	34/17	1.7 (0.9–3.2)	3.0 (0.9–9.9)	3.2 (1.2–8.8)
		P=0.13	P=0.09	P=0.04
IgG antibody to C. trachomatis
Negative	109/98	1.0	1.0	1.0
Positive	32/11	2.6 (1.3–5.4)	1.7 (0.7–4.3)	2.7 (1.3–6.0)
		P=0.01	P=0.26	P=0.01
Use of oral contraceptives
Never	130/98	1.0	1.0	1.0
Ever	7/9	0.6 (0.2–1.6)	0.9 (0.3–2.8)	0.6 (0.2–1.4)
		P=0.30	P=0.94	P=0.18
Age at first intercourse (years)
–23	111/74	1.9 (1.1–3.4)	1.8 (0.9–3.8)	1.8 (0.9–3.3)
24+	27/34	1.0	1.0	1.0
		P=0.03	P=0.14	P=0.10
Lifetime number of sexual partners
0–1	65/64	1.0	1.0	1.0
2–3	42/28	1.5 (0.8–2.7)	0.7 (0.2–1.8)	1.1 (0.5–2.4)
4+	34/17	2.0 (1.0–3.9)	2.2 (0.7–7.0)	2.0 (0.8–5.3)
		P for trend=0.04	P=0.41	P=0.15

Odds ratios.

95% confidence intervals.

IgG antibodies to any type of HPV16/52/58 L1-capsids.

DISCUSSION

With regard to smoking and C. trachomatis infection, the analysis adjusted for HPV antibodies gave different results from the analysis adjusted for HPV DNA. One explanation for this difference is that the antibody adjustment may reflect residual confounding by HPV, since only 54% of CIN cases were seropositive for HPV16/52/58 due to the limited sensitivity and high type-specificity of HPV capsid serology (Matsumoto ). Another reasonable explanation is that smoking and C. trachomatis infection may be significant cofactors associated with persistent HPV infections. In controls, HPV DNA positivity was much lower than seropositivity (15 vs 30%, respectively). Since HPV DNA testing at a single time point cannot identify past infections, the analysis based on HPV DNA may miss cofactors determining whether HPV infection is cleared or becomes persistent. In fact, a very recent study reported that smoking is associated with a reduced probability of clearing oncogenic HPV infections (Giuliano ). Associations of smoking and C. trachomatis infection with persistent HPV infections may be supported by several studies suggesting the modulation of host immunity by smoking (Barton ) and chlamydial infection (Zhong ).

Although the DNA adjustment enhanced the CIN risk among current smokers as well as the antibody adjustment, the association of smoking with an increased risk of CIN was not statistically significant in the DNA-based analysis. This may be due to the small numbers in the statistical analysis.

Unlike our previous study based on HPV DNA (Yoshikawa ), the present study has shown that smoking and C. trachomatis infection are significant cofactors for CIN development after adjustment for HPV antibodies. Simi-larly, associations of smoking and C. trachomatis infection with cervical neoplasia have been consistent in case–control studies employing HPV capsid serology (Dillner ; Olsen ; Kjellberg ; Koskela ; Anttila ), but inconsistent in HPV DNA-based studies (Schiffman ; de Sanjosé ; Eluf-Neto ; Olsen ; Ferrera ). Our observation suggests that epidemiological studies relying on a single assessment of HPV status should be interpreted with caution. To fully evaluate the role of epidemiological cofactors in HPV-related carcinogenesis, allowance for both HPV DNA and antibodies may be necessary.