J E Moore1, R McMullan. 1. Department of Bacteriology, Northern Ireland Public Health Laboratory, Belfast City Hospital, Northern Ireland. jemoore@niphl.dnet.co.uk
Abstract
BACKGROUND: Candida spp., mainly Candida albicans, are frequently responsible for complications in immunocompromised patients. There are limited data comparing recovery efficiency using simple non-selective basal broth media. AIM: To compare several commercially available basal growth media to determine the medium that gave highest yeast proliferation. METHOD: Eight commercially available non-selective basal broth culture media were evaluated for optimum recovery of clinical C. albicans and C. glabrata. They included nutrient broth (NB), nutrient broth no. 2 (NB2), Todd-Hewitt (TH) broth, tryptone soya broth (TSB), tryptone soya broth supplemented with yeast extract (0.5% w/v [TSBYE]), brain heart infusion broth supplemented with yeast extract (0.5% w/v [BHIYE]), salt meat broth (SMB) and 0.1% [w/v] peptone saline (PS). Differences in cell density were evaluated by spectrophotometrical analysis. RESULTS: TSBYE>BHIYE>TSB>TH>NB2>NB>SMB>PS for the optimum proliferation of cells in vitro. Either TSBYE or BHIYE broth may be employed as suitable basal broth media for growth of C. albicans and C. glabrata. NB should be considered the least suitable medium for routine use when others are available. CONCLUSION: These data may be of value to laboratories setting up simple blood culture systems to detect Candida spp., particularly in developing and underdeveloped countries.
BACKGROUND:Candida spp., mainly Candida albicans, are frequently responsible for complications in immunocompromised patients. There are limited data comparing recovery efficiency using simple non-selective basal broth media. AIM: To compare several commercially available basal growth media to determine the medium that gave highest yeast proliferation. METHOD: Eight commercially available non-selective basal broth culture media were evaluated for optimum recovery of clinical C. albicans and C. glabrata. They included nutrient broth (NB), nutrient broth no. 2 (NB2), Todd-Hewitt (TH) broth, tryptone soya broth (TSB), tryptone soya broth supplemented with yeast extract (0.5% w/v [TSBYE]), brain heart infusion broth supplemented with yeast extract (0.5% w/v [BHIYE]), salt meat broth (SMB) and 0.1% [w/v] peptone saline (PS). Differences in cell density were evaluated by spectrophotometrical analysis. RESULTS:TSBYE>BHIYE>TSB>TH>NB2>NB>SMB>PS for the optimum proliferation of cells in vitro. Either TSBYE or BHIYE broth may be employed as suitable basal broth media for growth of C. albicans and C. glabrata. NB should be considered the least suitable medium for routine use when others are available. CONCLUSION: These data may be of value to laboratories setting up simple blood culture systems to detect Candida spp., particularly in developing and underdeveloped countries.
Authors: C P Lindan; S Allen; A Serufilira; A R Lifson; P Van de Perre; A Chen-Rundle; J Batungwanayo; F Nsengumuremyi; J Bogaerts; S Hulley Journal: Ann Intern Med Date: 1992-02-15 Impact factor: 25.391