BACKGROUND: Expression of the inhibitor of apoptosis protein survivin is up-regulated in many tumors of epithelial origin and frequently shows a relationship with disease prognosis. MATERIALS AND METHODS: We investigated survivin mRNA expression in 32 urothelial cell carcinomas by use of real-time quantitative PCR. Expression values were normalized to transcript levels of the housekeeping gene cyclophilin. RESULTS: All bladder tumor tissues expressed survivin mRNA. The median normalized survivin mRNA expression values were 0.26 for superficial tumors (n = 17) and 0.78 for invasive tumors (n = 15). A significant relationship with increasing pathological stage (p < 0.001) and grade (p < 0.001) was observed. Although survivin mRNA expression did not relate to disease progression or the patient survival period, patients with superficial bladder tumors and normalized survivin values over 0.26 had an increased risk of recurrence (log-rank test: p = 0.018). CONCLUSION: Our results suggest that quantitative measurement of survivin mRNA 1) can identify invasive and high-grade urothelial cell carcinomas and 2) may be used as an indicator for early recurrence of superficial tumors.
BACKGROUND: Expression of the inhibitor of apoptosis protein survivin is up-regulated in many tumors of epithelial origin and frequently shows a relationship with disease prognosis. MATERIALS AND METHODS: We investigated survivin mRNA expression in 32 urothelial cell carcinomas by use of real-time quantitative PCR. Expression values were normalized to transcript levels of the housekeeping gene cyclophilin. RESULTS: All bladder tumor tissues expressed survivin mRNA. The median normalized survivin mRNA expression values were 0.26 for superficial tumors (n = 17) and 0.78 for invasive tumors (n = 15). A significant relationship with increasing pathological stage (p < 0.001) and grade (p < 0.001) was observed. Although survivin mRNA expression did not relate to disease progression or the patient survival period, patients with superficial bladder tumors and normalized survivin values over 0.26 had an increased risk of recurrence (log-rank test: p = 0.018). CONCLUSION: Our results suggest that quantitative measurement of survivin mRNA 1) can identify invasive and high-grade urothelial cell carcinomas and 2) may be used as an indicator for early recurrence of superficial tumors.
Authors: Minjian Cui; Jessie L-S Au; M Guillaume Wientjes; Michael A O'Donnell; Kevin R Loughlin; Ze Lu Journal: J Urol Date: 2015-02-11 Impact factor: 7.450
Authors: Marc Birkhahn; Anirban P Mitra; Anthony J Williams; Gitte Lam; Wei Ye; Ram H Datar; Marija Balic; Susan Groshen; Kenneth E Steven; Richard J Cote Journal: Eur Urol Date: 2009-09-09 Impact factor: 20.096
Authors: Doreen Kunze; Kai Kraemer; Kati Erdmann; Michael Froehner; Manfred P Wirth; Susanne Fuessel Journal: Int J Oncol Date: 2012-07-06 Impact factor: 5.650
Authors: Chun-Liang Chen; Ling Cen; Jennifer Kohout; Brian Hutzen; Christina Chan; Fu-Chuan Hsieh; Abbey Loy; Victor Huang; Gong Cheng; Jiayuh Lin Journal: Mol Cancer Date: 2008-10-21 Impact factor: 27.401
Authors: Anak A S S K Dharmapatni; Malcolm D Smith; David M Findlay; Christopher A Holding; Andreas Evdokiou; Michael J Ahern; Helen Weedon; Paul Chen; Gavin Screaton; Xiao N Xu; David R Haynes Journal: Arthritis Res Ther Date: 2009-01-27 Impact factor: 5.156