BACKGROUND AND AIMS: Recent molecular data suggest that genetic factors may underlie the disease heterogeneity observed in both ulcerative colitis (UC) and Crohn's disease (CD). A locus on chromosome 5q has been implicated in susceptibility to CD, and recently refined by linkage disequilibrium mapping to a conserved 250 kb haplotype (5q31). No data regarding the contribution of this locus to clinical phenotype exist. In this case control study, we investigated the contribution of this haplotype to both susceptibility and phenotype of CD and UC. PATIENTS AND METHODS: We studied 330 Caucasian CD and 457 UC patients recruited from a single UK centre. Association with disease susceptibility and phenotype was analysed with haplotypes reconstructed from three single nucleotide polymorphisms chosen to span this susceptibility region. Evidence for possible genetic epistasis between IBD5 and NOD2/CARD15 was sought. RESULTS: Linkage disequilibrium across this region was confirmed, with two haplotypes comprising 88% of all chromosomes. Susceptibility to CD, but not to UC, was associated with homozygosity for a common haplotype, H2 (p(c)=0.002; relative risk (RR) 2.0). Genotype-phenotype analyses demonstrated that this association was particularly strong in patients with perianal disease (p(c)=0.0005; RR 1.7), especially in individuals homozygous for this haplotype (p(c)=0.0005; RR 3.0). Importantly, no association with H2 was found in 186 patients without perianal disease. No evidence of epistasis between IBD5 and NOD2/CARD15 was demonstrated. CONCLUSIONS: The IBD5 risk haplotype is associated with CD only. Genotype-phenotype analysis reveals that the strongest association is observed in patients with perianal CD. While the precise gene involved is unclear, these data provide further molecular evidence for a genetic basis of the clinical heterogeneity of CD.
BACKGROUND AND AIMS: Recent molecular data suggest that genetic factors may underlie the disease heterogeneity observed in both ulcerative colitis (UC) and Crohn's disease (CD). A locus on chromosome 5q has been implicated in susceptibility to CD, and recently refined by linkage disequilibrium mapping to a conserved 250 kb haplotype (5q31). No data regarding the contribution of this locus to clinical phenotype exist. In this case control study, we investigated the contribution of this haplotype to both susceptibility and phenotype of CD and UC. PATIENTS AND METHODS: We studied 330 Caucasian CD and 457 UC patients recruited from a single UK centre. Association with disease susceptibility and phenotype was analysed with haplotypes reconstructed from three single nucleotide polymorphisms chosen to span this susceptibility region. Evidence for possible genetic epistasis between IBD5 and NOD2/CARD15 was sought. RESULTS: Linkage disequilibrium across this region was confirmed, with two haplotypes comprising 88% of all chromosomes. Susceptibility to CD, but not to UC, was associated with homozygosity for a common haplotype, H2 (p(c)=0.002; relative risk (RR) 2.0). Genotype-phenotype analyses demonstrated that this association was particularly strong in patients with perianal disease (p(c)=0.0005; RR 1.7), especially in individuals homozygous for this haplotype (p(c)=0.0005; RR 3.0). Importantly, no association with H2 was found in 186 patients without perianal disease. No evidence of epistasis between IBD5 and NOD2/CARD15 was demonstrated. CONCLUSIONS: The IBD5 risk haplotype is associated with CD only. Genotype-phenotype analysis reveals that the strongest association is observed in patients with perianal CD. While the precise gene involved is unclear, these data provide further molecular evidence for a genetic basis of the clinical heterogeneity of CD.
Authors: P Vavassori; P Borgiani; M R D'Apice; F De Negris; G Del Vecchio Blanco; I Monteleone; L Biancone; G Novelli; E Pallone Journal: Dig Liver Dis Date: 2002-02 Impact factor: 4.088
Authors: J D Rioux; M J Daly; M S Silverberg; K Lindblad; H Steinhart; Z Cohen; T Delmonte; K Kocher; K Miller; S Guschwan; E J Kulbokas; S O'Leary; E Winchester; K Dewar; T Green; V Stone; C Chow; A Cohen; D Langelier; G Lapointe; D Gaudet; J Faith; N Branco; S B Bull; R S McLeod; A M Griffiths; A Bitton; G R Greenberg; E S Lander; K A Siminovitch; T J Hudson Journal: Nat Genet Date: 2001-10 Impact factor: 38.330
Authors: G C Johnson; L Esposito; B J Barratt; A N Smith; J Heward; G Di Genova; H Ueda; H J Cordell; I A Eaves; F Dudbridge; R C Twells; F Payne; W Hughes; S Nutland; H Stevens; P Carr; E Tuomilehto-Wolf; J Tuomilehto; S C Gough; D G Clayton; J A Todd Journal: Nat Genet Date: 2001-10 Impact factor: 38.330
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Authors: J Hampe; A Cuthbert; P J Croucher; M M Mirza; S Mascheretti; S Fisher; H Frenzel; K King; A Hasselmeyer; A J MacPherson; S Bridger; S van Deventer; A Forbes; S Nikolaus; J E Lennard-Jones; U R Foelsch; M Krawczak; C Lewis; S Schreiber; C G Mathew Journal: Lancet Date: 2001-06-16 Impact factor: 79.321
Authors: Andrew P Cuthbert; Sheila A Fisher; Muddassar M Mirza; Kathy King; Jochen Hampe; Peter J P Croucher; Silvia Mascheretti; Jeremy Sanderson; Alastair Forbes; John Mansfield; Stefan Schreiber; Cathryn M Lewis; Christopher G Mathew Journal: Gastroenterology Date: 2002-04 Impact factor: 22.682
Authors: Maria Gazouli; Gerassimos Mantzaris; Athanassios J Archimandritis; George Nasioulas; Nicholas P Anagnou Journal: World J Gastroenterol Date: 2005-12-21 Impact factor: 5.742
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