| Literature DB >> 12848545 |
Satoshi Habuchi1, Mircea Cotlet, Roel Gronheid, Gunter Dirix, Jan Michiels, Jos Vanderleyden, Frans C De Schryver, Johan Hofkens.
Abstract
In the present contribution, we demonstrated that surface-enhanced resonance Raman scattering spectra from single green fluorescent proteins (GFPs) were obtained. The most important findings are the direct detection of the conversion between a deprotonated and a protonated form of the chromophore at the single-molecule level via the corresponding vibrational fingerprints, and the fact that the enhanced green fluorescent protein (EGFP) also shows a high surface enhanced resonance Raman scattering (SERRS) signal. Our findings show the potential of the technique to study structural dynamics of protein molecules at a single-molecule level.Entities:
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Year: 2003 PMID： 12848545 DOI： 10.1021/ja0353311
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419