Literature DB >> 12843096

Development of a duplex PCR assay for detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in pig feces.

Tom La1, Nyree D Phillips, David J Hampson.   

Abstract

A duplex PCR (D-PCR) amplifying portions of the Brachyspira hyodysenteriae NADH oxidase gene and the B. pilosicoli 16S rRNA gene was developed and then tested on DNA extracted from 178 porcine fecal samples. The feces also underwent anaerobic culture and species-specific PCRs. Fecal extraction-D-PCR detected seven additional samples containing B. hyodysenteriae and five more containing B. pilosicoli.

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Year:  2003        PMID： 12843096      PMCID： PMC165297          DOI： 10.1128/JCM.41.7.3372-3375.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

1.  Prevalence of Brachyspira pilosicoli in Korean pigs, determined using a nested PCR.

Authors:  C Choi; D U Han; J Kim; W S Cho; H K Chung; T Jung; B S Yoon; C Chae
Journal:  Vet Rec       Date:  2002-02-16       Impact factor: 2.695

2.  Development and evaluation of polymerase chain reaction tests as an aid to diagnosis of swine dysentery and intestinal spirochaetosis.

Authors:  R F Atyeo; S L Oxberry; B G Combs; D J Hampson
Journal:  Lett Appl Microbiol       Date:  1998-02       Impact factor: 2.858

3.  Specific detection of Serpulina hyodysenteriae and potentially pathogenic weakly beta-haemolytic porcine intestinal spirochetes by polymerase chain reaction targeting 23S rDNA.

Authors:  T D Leser; K Møller; T K Jensen; S E Jorsal
Journal:  Mol Cell Probes       Date:  1997-10       Impact factor: 2.365

4.  The porcine intestinal spirochaetes: identification of new genetic groups.

Authors:  J I Lee; D J Hampson; A J Lymbery; S J Harders
Journal:  Vet Microbiol       Date:  1993-03       Impact factor: 3.293

5.  Evidence for Serpulina hyodysenteriae being recombinant, with an epidemic population structure.

Authors:  Darren J Trott; Sophy L Oxberry; David J Hampson
Journal:  Microbiology (Reading)       Date:  1997-10       Impact factor: 2.777

6.  Identification of Serpulina species associated with porcine colitis by biochemical analysis and PCR.

Authors:  C Fellström; B Pettersson; J Thomson; A Gunnarsson; M Persson; K E Johansson
Journal:  J Clin Microbiol       Date:  1997-02       Impact factor: 5.948

7.  The use of culture, pooled samples and PCR for identification of herds infected with Brachyspira hyodysenteriae.

Authors:  C Fellström; U Zimmerman; A Aspan; A Gunnarsson
Journal:  Anim Health Res Rev       Date:  2001-06       Impact factor: 2.615

8.  Polymerase chain reaction for identification of human and porcine spirochaetes recovered from cases of intestinal spirochaetosis.

Authors:  N Y Park; C Y Chung; A J McLaren; R F Atyeo; D J Hampson
Journal:  FEMS Microbiol Lett       Date:  1995-01-15       Impact factor: 2.742

9.  Autoclaved liquid medium for propagation of Treponema hyodysenteriae.

Authors:  R A Kunkle; D L Harris; J M Kinyon
Journal:  J Clin Microbiol       Date:  1986-10       Impact factor: 5.948

10.  Rapid detection of Serpulina hyodysenteriae in diagnostic specimens by PCR.

Authors:  R O Elder; G E Duhamel; R W Schafer; M R Mathiesen; M Ramanathan
Journal:  J Clin Microbiol       Date:  1994-06       Impact factor: 5.948
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  23 in total

Review 1.  The Spirochete Brachyspira pilosicoli, Enteric Pathogen of Animals and Humans.

Authors:  David J Hampson
Journal:  Clin Microbiol Rev       Date:  2017-11-29       Impact factor: 26.132

2.  Intestinal spirochetosis due to Brachyspira pilosicoli: endoscopic and radiographic features.

Authors:  Junji Umeno; Takayuki Matsumoto; Shotaro Nakamura; Sohei Yoshino; Minako Hirahashi; Takashi Yao; Mitsuo Iida
Journal:  J Gastroenterol       Date:  2007-03-30       Impact factor: 7.527

3.  Brachyspira Species Avidity to Colonic Mucins from Pigs with and without Brachyspira hyodysenteriae Infection Is Species Specific and Varies between Strains.

Authors:  Macarena P Quintana-Hayashi; Mattias Erhardsson; Maxime Mahu; Vignesh Venkatakrishnan; Freddy Haesebrouck; Frank Pasmans; Sara Lindén
Journal:  Infect Immun       Date:  2021-09-20       Impact factor: 3.441

4.  Experimental Infection of Pigs with a ST 245 Brachyspira hyodysenteriae Isolated from an Asymptomatic Pig in a Herd with No History of Swine Dysentery.

Authors:  José Paulo H Sato; Amanda G S Daniel; Carlos E R Pereira; Mariana R Andrade; Ricardo P Laub; Michelle P Gabardo; Luisa V A Otoni; Nubia R Macedo; Javier A Barrera-Zarate; Roberto M C Guedes
Journal:  Vet Sci       Date:  2022-06-10

5.  Identification of Brachyspira hyodysenteriae and other pathogenic Brachyspira species in chickens from laying flocks with diarrhea or reduced production or both.

Authors:  Anneke Feberwee; David J Hampson; Nyree D Phillips; Tom La; Harold M J F van der Heijden; Gerard J Wellenberg; R Marius Dwars; Wil J M Landman
Journal:  J Clin Microbiol       Date:  2007-12-12       Impact factor: 5.948

6.  The use of ELISAs for monitoring exposure of pig herds to Brachyspira hyodysenteriae.

Authors:  Yong Song; Barbara Frey; David J Hampson
Journal:  BMC Vet Res       Date:  2012-01-17       Impact factor: 2.741

7.  The pathogenic intestinal spirochaete Brachyspira pilosicoli forms a diverse recombinant species demonstrating some local clustering of related strains and potential for zoonotic spread.

Authors:  Eugene Neo; Tom La; Nyree Dale Phillips; Mohammad Yousef Alikani; David J Hampson
Journal:  Gut Pathog       Date:  2013-08-16       Impact factor: 4.181

8.  Estimating diagnostic test accuracies for Brachyspira hyodysenteriae accounting for the complexities of population structure in food animals.

Authors:  Sonja Hartnack; Christina Nathues; Heiko Nathues; Elisabeth Grosse Beilage; Fraser Iain Lewis
Journal:  PLoS One       Date:  2014-06-06       Impact factor: 3.240

9.  The prevalence of swine enteropathogens in Brazilian grower and finish herds.

Authors:  A M Viott; A P Lage; E C C Cruz; R M C Guedes
Journal:  Braz J Microbiol       Date:  2013-05-17       Impact factor: 2.476

10.  Absence of a set of plasmid-encoded genes is predictive of reduced pathogenic potential in Brachyspira hyodysenteriae.

Authors:  Tom La; Nyree D Phillips; Jill R Thomson; David J Hampson
Journal:  Vet Res       Date:  2014-12-16       Impact factor: 3.683
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