BACKGROUND: Lactobacillus crispatus is a part of the normal vaginal microflora of humans. GOAL: The goal of this study was to assess whether a capsule containing an H2O2-producing strain of L crispatus (CTV-05) would alter the vaginal microflora and/or epithelial tissues when applied intravaginally in the pig-tailed macaque model. STUDY DESIGN: Ten sexually mature female Macaca nemestrina were assessed at baseline for quantitative vaginal microbiology and vaginal pH and with colposcopy. One capsule containing 108 colony forming units of desiccated L crispatus CTV-05 was inserted into the vaginal fornix of each animal. Vaginal assessments were repeated on days 1 and 2 after capsule insertion. The L crispatus CTV-05 strain was identified with use of a DNA fingerprinting method. RESULTS: Before product use, four of 10 animals had detectable levels of H2O2-producing lactobacilli. L crispatus CTV-05 was detected in 1 of 10 animals on day 1 and in 3 of 10 animals on day 2 following insertion of the capsule. There were no tissue changes observed by colposcopy. Vaginal pH decreased in two animals colonized by CTV-05, from 7.0 at baseline to 4.5+/-0.5 on days 1 and 2 after product use. CONCLUSIONS: A single intravaginal application of capsules containing 108 L crispatus CTV-05 resulted in vaginal colonization in three of 10 animals 2 days after use. The absence of colposcopic changes in the vagina/cervical tissues indicates that L crispatus capsules are well tolerated.
BACKGROUND:Lactobacillus crispatus is a part of the normal vaginal microflora of humans. GOAL: The goal of this study was to assess whether a capsule containing an H2O2-producing strain of L crispatus (CTV-05) would alter the vaginal microflora and/or epithelial tissues when applied intravaginally in the pig-tailed macaque model. STUDY DESIGN: Ten sexually mature female Macaca nemestrina were assessed at baseline for quantitative vaginal microbiology and vaginal pH and with colposcopy. One capsule containing 108 colony forming units of desiccated L crispatus CTV-05 was inserted into the vaginal fornix of each animal. Vaginal assessments were repeated on days 1 and 2 after capsule insertion. The L crispatus CTV-05 strain was identified with use of a DNA fingerprinting method. RESULTS: Before product use, four of 10 animals had detectable levels of H2O2-producing lactobacilli. L crispatus CTV-05 was detected in 1 of 10 animals on day 1 and in 3 of 10 animals on day 2 following insertion of the capsule. There were no tissue changes observed by colposcopy. Vaginal pH decreased in two animals colonized by CTV-05, from 7.0 at baseline to 4.5+/-0.5 on days 1 and 2 after product use. CONCLUSIONS: A single intravaginal application of capsules containing 108 L crispatus CTV-05 resulted in vaginal colonization in three of 10 animals 2 days after use. The absence of colposcopic changes in the vagina/cervical tissues indicates that L crispatus capsules are well tolerated.
Authors: Xiaowen Liu; Laurel A Lagenaur; David A Simpson; Kirsten P Essenmacher; Courtney L Frazier-Parker; Yang Liu; Daniel Tsai; Srinivas S Rao; Dean H Hamer; Thomas P Parks; Peter P Lee; Qiang Xu Journal: Antimicrob Agents Chemother Date: 2006-10 Impact factor: 5.191
Authors: Jeanne M Marrazzo; David H Martin; D Heather Watts; Joann Schulte; Jack D Sobel; Sharon L Hillier; Carolyn Deal; David N Fredricks Journal: Sex Transm Dis Date: 2010-12 Impact factor: 2.830
Authors: Alexander M Cole; Dorothy L Patton; Lisa C Rohan; Amy L Cole; Yvonne Cosgrove-Sweeney; Nicole A Rogers; Deena Ratner; Alexandra B Sassi; Carol Lackman-Smith; Patrick Tarwater; Bharat Ramratnam; Piotr Ruchala; Robert I Lehrer; Alan J Waring; Phalguni Gupta Journal: PLoS One Date: 2010-11-29 Impact factor: 3.240
Authors: Elizabeth Neuendorf; Pawel Gajer; Anne K Bowlin; Patricia X Marques; Bing Ma; Hongqiu Yang; Li Fu; Michael S Humphrys; Larry J Forney; Garry S A Myers; Patrik M Bavoil; Roger G Rank; Jacques Ravel Journal: Pathog Dis Date: 2015-03-10 Impact factor: 3.166