Literature DB >> 12837806

An integrated structural and computational study of the thermostability of two thioredoxin mutants from Alicyclobacillus acidocaldarius.

Simonetta Bartolucci1, Giuseppina De Simone, Stefania Galdiero, Roberto Improta, Valeria Menchise, Carlo Pedone, Emilia Pedone, Michele Saviano.   

Abstract

We report a crystallographic and computational analysis of two mutant forms of the Alicyclobacillus acidocaldarius thioredoxin (BacTrx) done in order to evaluate the contribution of two specific amino acids to the thermostability of BacTrx. Our results suggest that the thermostability of BacTrx may be modulated by mutations affecting the overall electrostatic energy of the protein.

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Year:  2003        PMID： 12837806      PMCID： PMC164891          DOI： 10.1128/JB.185.14.4285-4289.2003

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  36 in total

1.  Increasing protein stability by altering long-range coulombic interactions.

Authors:  G R Grimsley; K L Shaw; L R Fee; R W Alston; B M Huyghues-Despointes; R L Thurlkill; J M Scholtz; C N Pace
Journal:  Protein Sci       Date:  1999-09       Impact factor: 6.725

2.  Engineering a thermostable protein via optimization of charge-charge interactions on the protein surface.

Authors:  V V Loladze; B Ibarra-Molero; J M Sanchez-Ruiz; G I Makhatadze
Journal:  Biochemistry       Date:  1999-12-14       Impact factor: 3.162

3.  Single surface stabilizer.

Authors:  C N Pace
Journal:  Nat Struct Biol       Date:  2000-05

4.  Structural differences between mesophilic, moderately thermophilic and extremely thermophilic protein subunits: results of a comprehensive survey.

Authors:  A Szilágyi; P Závodszky
Journal:  Structure       Date:  2000-05-15       Impact factor: 5.006

5.  Contribution of surface salt bridges to protein stability.

Authors:  P Strop; S L Mayo
Journal:  Biochemistry       Date:  2000-02-15       Impact factor: 3.162

6.  Thermal adaptation analyzed by comparison of protein sequences from mesophilic and extremely thermophilic Methanococcus species.

Authors:  P J Haney; J H Badger; G L Buldak; C I Reich; C R Woese; G J Olsen
Journal:  Proc Natl Acad Sci U S A       Date:  1999-03-30       Impact factor: 11.205

7.  The hyperthermostable indoleglycerol phosphate synthase from Thermotoga maritima is destabilized by mutational disruption of two solvent-exposed salt bridges.

Authors:  A Merz; T Knöchel; J N Jansonius; K Kirschner
Journal:  J Mol Biol       Date:  1999-05-14       Impact factor: 5.469

8.  Rational modification of protein stability by the mutation of charged surface residues.

Authors:  S Spector; M Wang; S A Carp; J Robblee; Z S Hendsch; R Fairman; B Tidor; D P Raleigh
Journal:  Biochemistry       Date:  2000-02-08       Impact factor: 3.162

9.  NMR solution structure of a novel thioredoxin from Bacillus acidocaldarius possible determinants of protein stability.

Authors:  G Nicastro; C De Chiara; E Pedone; M Tatò; M Rossi; S Bartolucci
Journal:  Eur J Biochem       Date:  2000-01

10.  Prediction and experimental testing of Bacillus acidocaldarius thioredoxin stability.

Authors:  E Pedone; R Cannio; M Saviano; M Rossi; S Bartolucci
Journal:  Biochem J       Date:  1999-04-15       Impact factor: 3.857
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  2 in total

1.  On the origin of life in the zinc world. 2. Validation of the hypothesis on the photosynthesizing zinc sulfide edifices as cradles of life on Earth.

Authors:  Armen Y Mulkidjanian; Michael Y Galperin
Journal:  Biol Direct       Date:  2009-08-24       Impact factor: 4.540

2.  Expression, purification and X-ray crystallographic analysis of thioredoxin from Streptomyces coelicolor.

Authors:  Petra Stefankova; Jana Maderova; Imrich Barak; Marta Kollarova; Zbyszek Otwinowski
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2005-01-08
  2 in total

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